Compositions, methods, and kits for delivery of polyribonucleotides
AssigneeFlagship Pioneering Innovations VI, LLC
InventorsAvak KAHVEJIAN, Nicholas McCartney PLUGIS, Alexandra Sophie DE BOER, Ellese Marie CARMONA, Morag Helen STEWART, Roger Joseph HAJJAR
Life of this patent
Estimating the term…
Abstract
Disclosed herein are methods and compositions for delivery of a polyribonucleotide. In some cases, the methods and compositions provided herein are suitable for in vivo delivery of polyribonucleotides. In certain aspects, also disclosed herein are pharmaceutical compositions for delivery of polyribonucleotide having biological effects.
Claims34
A pharmaceutical composition comprising a mixture of a polyribonucleotide and ethanol, wherein the ethanol constitutes:
- (i) at least about 0.3% v/v to about 75% v/v of the mixture;
- or
- (ii) at least about 0.3% v/v to about 70% v/v, at least about 0.3% v/v to about 60% v/v, at least about 0.3% v/v to about 50% v/v, at least about 0.3% v/v to about 40% v/v, at least about 30% v/v to about 20% v/v, at least about 0.3% v/v to about 15% v/v, at least about 0.3% v/v to about 10% v/v, at least about 0.3% v/v to about 5% v/v, at least about 0.3% v/v to about 1% v/v, or at least about 0.3% v/v to about 0.5% v/v of the mixture;
- or
- (iii) at least about 0.5% v/v to about 75% v/v, at least about 1% v/v to about 75% v/v, at least about 5% v/v to about 75% v/v, at least about 10% v/v to about 75% v/v, at least about 15% v/v to about 75% v/v, at least about 20% v/v to about 75% v/v, at least about 30% v/v to about 75% v/v, at least about 40% v/v to about 75% v/v, at least about 50% v/v to about 75% v/v, at least about 60% v/v to about 75% v/v, or at least about 70% v/v to about 75% v/v of the mixture.
A method of delivering a polyriboribonucleotide to a subject comprising topically applying a composition comprising a mixture of a polyribonucleotide and ethanol to a surface area of the subject, wherein the ethanol constitutes at least about 0.3% v/v to about 75% v/v of the mixture.
A method of delivering a polyribonucleotide to a subject comprising a) applying a sterilizing agent to a surface area of the subject;
- b) applying a composition free of any carrier comprising the polyribonucleotide and diluent to the surface area.
A method of delivering a polyribonucleotide to a subject comprising
- a) applying an alcohol to a surface area of the subject;
- b) applying a composition free of any carrier comprising the polyribonucleotide and diluent to the surface.
A method of delivering a polyribonucleotide to an epithelial cell comprising applying a composition free of any carrier comprising a diluent and a polyribonucleotide that is not modified to the epithelial cell.
A method of delivering a polyribonucleotide to a subject comprising topically applying a composition comprising a mixture of a polyribonucleotide and an alcohol to a surface area of the subject, wherein the alcohol constitutes at least about 0.3% v/v to about 75% v/v of the mixture.
A method of delivering a polyribonucleotide to a subject comprising topically applying a composition comprising a mixture of a polyribonucleotide and a cell-penetrating agent to a surface area of the subject, wherein the cell-penetrating agent constitutes at least about 0.3% v/v to about 75% v/v of the mixture.
A kit comprising a first application tool, a second application tool, a sterilizing agent, and a composition free of any carrier comprising the polyribonucleotide and diluent, wherein the first application tool is configured to apply a sterilizing agent to a surface area of a subject and the second application tool is configured to apply the composition to the surface area of the subject.
A kit comprising an application tool and a mixture comprising a polyribonucleotide and a cell-penetrating agent, wherein the application tool is configured to apply the mixture to a surface area of a subject.
A pharmaceutical composition comprising a mixture of a polyribonucleotide and an alcohol, wherein the alcohol constitutes:
- (i) at least about 0.3% v/v to about 75% v/v of the mixture;
- or
- (ii) at least about 0.3% v/v to about 70% v/v, at least about 0.3% v/v to about 60% v/v, at least about 0.3% v/v to about 50% v/v, at least about 0.3% v/v to about 40% v/v, at least about 30% v/v to about 20% v/v, at least about 0.3% v/v to about 15% v/v, at least about 0.3% v/v to about 10% v/v, at least about 0.3% v/v to about 5% v/v, or at least about 0.3% v/v to about 1% v/v, or at least about 0.3% v/v to about 0.5% v/v of the mixture;
- or
- (iii) at least about 0.5% v/v to about 75% v/v, at least about 1% v/v to about 75% v/v, at least about 5% v/v to about 75% v/v, at least about 10% v/v to about 75% v/v, at least about 15% v/v to about 75% v/v, at least about 20% v/v to about 75% v/v, at least about 30% v/v to about 75% v/v, at least about 40% v/v to about 75% v/v, at least about 50% v/v to about 75% v/v, at least about 60% v/v to about 75% v/v, or at least about 70% v/v to about 75% v/v of the mixture.
A pharmaceutical composition comprising a mixture of a polyribonucleotide and a cell-penetrating agent, wherein the cell-penetrating agent constitutes:
- (i) at least about 0.3% v/v to about 75% v/v of the mixture;
- or
- (ii) at least about 0.3% v/v to about 70% v/v, at least about 0.3% v/v to about 60% v/v, at least about 0.3% v/v to about 50% v/v, at least about 0.3% v/v to about 40% v/v, at least about 30% v/v to about 20% v/v, at least about 0.3% v/v to about 15% v/v, at least about 0.3% v/v to about 10% v/v, at least about 0.3% v/v to about 5% v/v, at least about 0.3% v/v to about 1% v/v, or at least about 0.3% v/v to about 0.5% v/v of the mixture;
- or
- (iii) at least about 0.5% v/v to about 75% v/v, at least about 1% v/v to about 75% v/v, at least about 5% v/v to about 75% v/v, at least about 10% v/v to about 75% v/v, at least about 15% v/v to about 75% v/v, at least about 20% v/v to about 75% v/v, at least about 30% v/v to about 75% v/v, at least about 40% v/v to about 75% v/v, at least about 50% v/v to about 75% v/v, at least about 60% v/v to about 75% v/v, or at least about 70% v/v to about 75% v/v of the mixture.
Dependencies are inferred from the claim text.