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US 2022/0087306 A1Published application
Reducing levels of nicotinic alkaloids in plants
Assignee22nd Century Limited, LLC
InventorsTakashi HASHIMOTO, Akira KATO
Life of this patent
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Abstract
Two genes, A622 and NBB1, can be influenced to achieve a decrease of nicotinic alkaloid levels in plants. In particular, suppression of one or both of A622 and NBB1 may be used to decrease nicotine in tobacco plants.
Claims11
-27. (canceled)
A method for producing a tobacco plant comprising:
- (a) mutagenizing a plurality of tobacco plant cells to comprise one or more mutations in the nucleotide sequence set forth in SEQ ID NO:
- 1, thereby producing a plurality of mutagenized cells comprising the one or more mutations in the nucleotide sequence set forth in SEQ ID NO:
- 1;
- (b) screening plants generated from the mutagenized cells comprising the one or more mutations in the nucleotide sequence set forth in SEQ ID NO:
- 1 for reduced expression of A622;
- and
- (c) selecting one or more of the progeny comprising the one or more mutations in the nucleotide sequence set forth in SEQ ID NO:
- 1, wherein the progeny has reduced A622 expression and reduced anatabine content as compared to a control tobacco plant,
- thereby resulting in a tobacco plant having reduced A622 expression and reduced anatabine content as compared to a control tobacco plant.
6 more elements
A method for producing a reduced-anatabine tobacco plant comprising:
- introducing into a plant cell of the tobacco plant a nucleic acid construct that expresses a short interfering RNA that suppresses expression of a gene product encoded by SEQ ID NO:
- 1;
- thereby resulting in a tobacco plant exhibiting suppression of a gene product encoded by SEQ ID NO:
- 1 and reduced anatabine content as compared to a non-transformed control plant.
1 more element
Dependencies are inferred from the claim text.