Protein methods and compositions
AssigneeImpossible Foods Inc.
InventorsCarl Allen Henderson, Jr., Ratnayake Mudiyanselage Dunilka Nishani Ratnayake, Ian Guiles Ronningen, Laila Dafik
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Abstract
This disclosure describes methods for purifying protein, and more particularly to methods for purifying protein that minimize the development of undesirable odors and flavors in the purified protein and increase protein yield.
Claims26
A method for purifying proteins from a plurality of cells having cell walls, the method comprising:
- a) perforating the cell walls of the plurality of cells;
- b) separating an aqueous suspension of the plurality of cells to form a solids portion and a liquid portion;
- c) filtering the liquid portion to form a filtrate and a retentate;
- d) concentrating the retentate to form a protein composition;
- and
- e) optionally pasteurizing the protein composition,
- wherein each of a)-d), independently, are performed at a pH of about 8.5 and about 12.0, and/or wherein the method results in the liquid portion comprising at least about 50% by weight of the cytoplasmic proteins of the plurality of cells.
A method for purifying proteins from a plurality of cells, the method comprising:
- a) treating an aqueous suspension of the plurality of cells with a base until the pH of the aqueous suspension is about 8.5 to about 12.0;
- b) separating the aqueous suspension of the plurality of cells to form a solids portion and a liquid portion;
- c) filtering the liquid portion to form a filtrate and a retentate;
- d) concentrating the retentate to form a protein composition;
- and
- e) optionally pasteurizing the protein composition,
- wherein each of a)-d), independently, are performed at a pH of about 8.5 and about 12.0, and/or wherein the method results in the liquid portion comprising at least about 10% by weight of the cytoplasmic proteins of the plurality of cells.
3-4. (canceled)
A method for purifying proteins from a plurality of cells, the method comprising:
- a) heating the plurality of cells to a temperature of about 50° C. to about 85° C.;
- b) separating an aqueous suspension of the plurality of cells to form a solids portion and a liquid portion;
- c) filtering the liquid portion to form a filtrate and a retentate;
- d) concentrating the retentate to form a protein composition;
- and
- e) optionally pasteurizing the protein composition,
- wherein each of a)-d), independently, are performed at a pH of about 8.5 and about 12.0, and/or wherein the method results in the liquid portion comprising at least about 50% by weight of the cytoplasmic proteins of the plurality of cells.
6-9. (canceled)
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13-15. (canceled)
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32-60. (canceled)
Dependencies are inferred from the claim text.