Process for purifying a human milk oligosaccharide and related compositions
AssigneeDupont Nutrition Biosciences Aps
InventorsHannu KOIVIKKO, Juho JÄRVINEN
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Abstract
This specification relates to preparing a purified human milk oligosaccharide (HMO) from an HMO solution by a process comprising nanofiltration, as well as processes for making foods, dietary supplements, medicines and infant formulas comprising a purified HMO. This specification also relates to purified HMOs and foods, dietary supplements, medicines and infant formulas prepared by processes disclosed in this specification.
Claims31
A process for preparing a purified human milk oligosaccharide (HMO), wherein:
- the process comprises:
- feeding an HMO solution into a nanofiltration unit,
- filtrating the HMO solution using the nanofiltration unit to produce a concentrate and a permeate, and
- collecting the concentrate;
- the HMO solution comprises an aqueous medium comprising an HMO to be purified and a second carbohydrate;
- the nanofiltration unit comprises a membrane having:
- an MgSO4 retention of at least about 50% (at 25° C., 2 g/l concentration, 8 bar and pH 6-7), and
- an NaCl retention of up to about 60% (at 25° C., 2 g/l concentration, 8 bar and pH 6-7);
- and
- the filtrating is carried out at a temperature of less than 10° C.
A process for preparing a human milk oligosaccharide (HMO), wherein:
- the process comprises:
- feeding an HMO solution into a nanofiltration unit,
- filtrating the HMO solution using the nanofiltration unit to produce a concentrate and a permeate, and
- collecting the concentrate;
- the HMO solution comprises an aqueous medium comprising an HMO to be purified and a second carbohydrate;
- the nanofiltration unit comprises a membrane having:
- an MgSO4 retention of greater than 90% (at 25° C., 2 g/l concentration, 8 bar and pH 6-7), and
- an NaCl retention of up to about 60% (at 25° C., 2 g/l concentration, 8 bar and pH 6-7);
- and
- the filtrating is carried out at a temperature of no greater than about 18° C.
A process for preparing a purified human milk oligosaccharide (HMO), wherein:
- the process comprises:
- feeding an HMO solution into a nanofiltration unit,
- filtrating the HMO solution using the nanofiltration unit to produce a concentrate and a permeate, and
- collecting the concentrate;
- the HMO solution comprises an aqueous medium comprising an HMO to be purified and a second carbohydrate;
- the nanofiltration unit comprises a membrane having:
- an MgSO4 retention of at least about 50% (at 25° C., 2 g/l concentration, 8 bar and pH 6-7), and
- an NaCl retention of up to about 60% (at 25° C., 2 g/l concentration, 8 bar and pH 6-7);
- the nanofiltration unit comprises a membrane having a molecular weight cut off of less than 600 Dalton;
- and
- the filtrating is carried out at a temperature of no greater than about 18° C.
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The process according to, wherein the HMO to be purified is 2′-fucosyllactose, 3-fucosyllactose, 3′-sialyllactose, 6′-sialyllactose or lacto-N-tetraose.
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Dependencies are inferred from the claim text.