Use of sideromycins to limit cross-reactivity and improve species identification in antibiotic susceptibility assays
AssigneeRoche Molecular Systems, Inc.
InventorsKyle C. Cady, Ryan Chan
Life of this patent
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Abstract
The present invention relates to the use of sideromycins as additives in non-replicative transduction particles based systems either to limit cross-reactivity of unwanted organisms or to identify the organism being run on an antibiotic susceptibility assay (AST assay). Addition of the sideromycins removes or reduces light production from bacteria that are sensitive to them, allowing for prevention of cross-reactivity in AST assays and/or family, genus, and potentially species level bacteria strain identification when performing AST testing.
Claims15
A method for reducing the amount of potentially cross-reactive or interfering organisms in an assay designed to detect a detectable indication of viability of a target organism, comprising:
- obtaining a sample potentially comprising at least one organism that is potentially cross-reactive or interfering in an assay designed to detect the detectable indication of viability of the target organism;
- contacting the cross-reactive or interfering organism with at least one compound that is involved with the viability of the potentially cross-reactive or interfering organism, wherein the compound is specific to the cross-reactive or interfering organism and causing the cross-reactive or interfering organism to lose viability without affecting viability of the target organism;
- contacting the sample with a non-replicative transduction particle (NRTP) comprising a reporter nucleic acid molecule encoding a reporter molecule, under conditions such that the NRTP inserts into the target organism the reporter nucleic acid molecule and such that the reporter molecule provides the detectable indication of viability of the target organism.
A method of classifying a microorganism as Enterobacteriaceae in origin or non-Enterobacteriacea in origin, comprising:
- obtaining a sample containing said microorganism;
- contacting said sample with a sideromycin combination comprising Albomycin and SalmycinA;
- contacting said sample with a non-replicative transduction particle (NRTP) comprising a reporter nucleic acid molecule encoding a reporter molecule, under conditions such that the NRTP inserts into the microorganism the reporter nucleic acid molecule and such that the reporter molecule provides a detectable indication of viability of the microorganism;
- wherein said microorganism is classified as Enterobacteriaceae in origin if the detectable indication of viability of the microorganism is reduced by greater than 50% from the presence of the sideromycin combination, and wherein said microorganism is classified as non-Enterobacteriaceae in origin if the detectable indication of viability of the microorganism is reduced by less than 50% from the presence of the sideromycin combination.
A kit for reducing the amount of potentially cross-reactive or interfering organisms in an assay designed to detect a target organism comprising:
- a compound that causes the cross-reactive or interfering organism to lose viability without affecting viability of the target organism;
- and
- a non-replicative transduction particle (NRTP) comprising a reporter nucleic acid molecule encoding a reporter molecule, under conditions such that the NRTP inserts into the target organism the reporter nucleic acid molecule and such that the reporter molecule provides a detectable indication of viability of the target organism.
Dependencies are inferred from the claim text.