Recombinant yeast and method for producing ethanol using same
AssigneeToyota Jidosha Kabushiki Kaisha
InventorsRie HIRAO, Nobuki TADA, Toru ONISHI
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Abstract
Provided are excellent L-arabinose metabolic genes that function in yeasts. Provided is an L-arabinose metabolic gene cluster including an L-arabinose isomerase gene specified by a predetermined SEQ ID, an L-ribulokinase gene specified by a predetermined SEQ ID, and an L-ribulose-5-phosphate-4-epimerase gene specified by a predetermined SEQ ID.
Claims9
A recombinant yeast comprising a group L-arabinose metabolic genes including an L-arabinose isomerase gene, an L-ribulokinase gene, and an L-ribulose-5-phosphate-4-epimerase gene introduced thereinto, wherein
- the L-arabinose isomerase gene is a gene encoding any one of proteins (a) to (c) below:
- (a) a protein comprising one amino acid sequence selected from the group consisting of SEQ ID NOs:
- 2, 4, and 6;
- (b) a protein comprising an amino acid sequence having an identity of 80% or more to one amino acid sequence selected from the group consisting of SEQ ID NOs:
- 2, 4, and 6 and having L-arabinose isomerase activity;
- and
- (c) a protein encoded by a nucleotide sequence that hybridizes with a nucleotide sequence complementary to one nucleotide sequence selected from the group consisting of SEQ ID NOs:
- 1, 3, and 5 under stringent conditions and having L-arabinose isomerase activity.
A recombinant yeast comprising a group of L-arabinose metabolic genes including an L-arabinose isomerase gene, an L-ribulokinase gene, and an L-ribulose-5-phosphate-4-epimerase gene introduced thereinto, wherein
- the L-ribulokinase gene is a gene encoding any one of proteins (a) to (c) below:
- (a) a protein comprising one amino acid sequence selected from the group consisting of SEQ ID NOs:
- 8, 10, 12, 14, and 16;
- (b) a protein comprising an amino acid sequence having an identity of 80% or more to one amino acid sequence selected from the group consisting of SEQ ID NOs:
- 8, 10, 12, 14, and 16 and having L-ribulokinase activity;
- and
- (c) a protein encoded by a nucleotide sequence that hybridizes with a nucleotide sequence complementary to one nucleotide sequence selected from the group consisting of SEQ ID NOs:
- 7, 9, 11, 13, and 15 under stringent conditions and having L-ribulokinase activity.
A recombinant yeast comprising a group of L-arabinose metabolic genes including an L-arabinose isomerase gene, an L-ribulokinase gene, and an L-ribulose-5-phosphate-4-epimerase gene introduced thereinto, wherein
- the L-ribulose-5-phosphate-4-epimerase gene is a gene encoding any one of proteins (a) to (c) below:
- (a) a protein comprising one amino acid sequence selected from the group consisting of SEQ ID NOs:
- 18, 20, and 22;
- (b) a protein comprising an amino acid sequence having an identity of 80% or more to one amino acid sequence selected from the group consisting of SEQ ID NOs:
- 18, 20, and 22 and having L-ribulose-5-phosphate-4-epimerase activity;
- and
- (c) a protein encoded by a nucleotide sequence that hybridizes with a nucleotide sequence complementary to one nucleotide sequence selected from the group consisting of SEQ ID NOs:
- 17, 19, and 21 under stringent conditions and having L-ribulose-5-phosphate-4-epimerase activity.
Dependencies are inferred from the claim text.