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AssigneesPolitecnico Di Milano, Universita' Degli Studi Di Pavia, Universita' Degli Studi Di Torino
InventorsPaola PETRINI, Daniela Patricia PENEDA PACHECO, Natalia Andrea SUAREZ VARGAS, Federico BERTOGLIO, Sonja VISENTIN, Livia VISAI
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A three-dimensional substrate with structural and compositional gradient for microbial cultures and cocultures is provided. The three-dimensional substrate includes a diffusion system having a first apical compartment placed above a second basolateral compartment, the first and second compartments being separated by a semipermeable membrane, a base solution comprising polysaccharides, proteins and salts or a preformed hydrogel comprising polysaccharides, proteins and salts, and a cross-linking medium comprising salts, culture media and distilled water. A method for preparing a three-dimensional substrate with structural and compositional gradient for microbial cultures and cocultures is also provided.
A diffusion system for preparing a microbial growth substrate with modular cross-linking gradient, the diffusion system comprising a first apical compartment and a second basolateral compartment, wherein said first apical compartment is placed above said second basolateral compartment, wherein a base solution comprising polysaccharides, proteins and salts is contained in said first apical compartment and wherein a cross-linking medium comprising salts, culture media and distilled water is contained in said second basolateral compartment, and wherein said first apical compartment and second basolateral compartment are separated by a semipermeable membrane, which is permeable to salts but impermeable to polysaccharides and proteins.
A method for preparing a three-dimensional substrate for microbial cultures, the method comprising:
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