Uterine endometrial fluid for prediction of success in fertility treatment
AssigneeFertility Lab Sciences, LLC
InventorsMandy Katz-Jaffe, William B. Schoolcraft
Life of this patent
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Abstract
Provided herein are methods, systems, and kits for improving success rates in assisted reproductive technologies such as in vitro fertilization, frozen embryo transfer, and intrauterine insemination. These methods, systems, and kits rely on levels of protein, metabolite, and microRNA markers determined herein to be linked to uterine toxicity and embryo implantation failure.
Claims41
A method of predicting negative pregnancy outcome in fertility treatment, the method comprising:
- contacting uterine endometrial secretions from a patient with a kit that comprises a solid-state substrate functionalized to identify one or more, or at least two, markers associated with a hostile endometrial environment selected from the group consisting of proteins, metabolites, or miRNAs, and
- determining the secretome profile of the uterine endometrial secretions to ascertain an increase or decrease in the presence of markers associated with a hostile endometrial environment,
- wherein the increase or decrease in the presence of one or more markers associated with a hostile endometrial environment, relative to the secretome profile of uterine endometrial secretions of a successful pregnancy outcome, predicts negative pregnancy outcome.
A kit comprising a solid-state substrate functionalized to identify one or more, or at least two, markers associated with a hostile endometrial environment selected from the group consisting of proteins, metabolites, and miRNAs.
A system for enhancing the pregnancy success rate of fertility treatment, comprising predicting a negative pregnancy outcome in a patient undergoing fertility treatment prior to frozen embryo transfer or intrauterine insemination, wherein the predicting comprises determining the secretome profile of the patient's uterine endometrial secretions to ascertain an increase or decrease in the presence of markers associated with a hostile endometrial environment,
- wherein the increase or decrease in the presence of one or more markers associated with a hostile endometrial environment, relative to the secretome profile of uterine endometrial secretions of a successful pregnancy outcome, predicts negative pregnancy outcome in the patient.
An in vitro method of screening a fertility patient prior to frozen embryo transfer or intrauterine insemination, the method comprising contacting uterine endometrial secretions from a patient with a kit that comprises a solid-state substrate functionalized to identify one or more, or at least two, markers associated with a hostile endometrial environment selected from the group consisting of proteins, metabolites, or miRNAs, and
- determining the secretome profile of the uterine endometrial secretions to ascertain an increase or decrease in the presence of markers associated with a hostile endometrial environment,
- wherein the increase or decrease in the presence of one or more markers associated with a hostile endometrial environment, relative to the secretome profile of uterine endometrial secretions of a successful pregnancy outcome, predicts negative pregnancy outcome.
A method of predicting implantation failure of a candidate embryo, the method comprising:
- contacting uterine endometrial secretions from a patient with a kit that comprises a solid-state substrate functionalized to identify one or more, or at least two, markers associated with a hostile endometrial environment selected from the group consisting of proteins, metabolites, or miRNAs, and
- determining the secretome profile of the uterine endometrial secretions to ascertain an increase or decrease in the presence of markers associated with a hostile endometrial environment,
- wherein the increase or decrease in the presence of one or more markers associated with a hostile endometrial environment, relative to the secretome profile of uterine endometrial secretions of a successful pregnancy outcome, predicts embryo implantation failure.
A method of treating female infertility comprising:
- (a) ascertaining miR-17 levels in the uterine endometrial secretions from a patient,
- (b) treating a patient having an increase in miR-17 levels in the uterine endometrial secretions relative to a miR-17 profile indicative of a successful pregnancy outcome with recombinant human VEGF-A;
- and
- (c) performing frozen embryo transfer or intrauterine insemination.
Dependencies are inferred from the claim text.