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US 2022/0065856 A1Published application
Affinity-oligonucleotide conjugates and uses thereof
AssigneeAbVitro LLC
InventorsFrancois Vigneault, Adrian Wrangham Briggs, Stephen J. Goldfless, Brian J. Belmont
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Abstract
Provided herein are methods and compositions for single cell characterization using affinity-oligonucleotide conjugates.
Claims3
1-124. (canceled)
A kit for making conjugates, comprising:
- (a) a first container comprising a first oligonucleotide comprising a first antigen identification (AID) sequence and a first affinity molecular barcode (AMB) sequence, wherein the first AID sequence and the first AMB sequence are known sequences;
- (b) a second container comprising a second oligonucleotide comprising a second antigen identification (AID) sequence and a second affinity molecular barcode (AMB) sequence, wherein the second AID sequence is a known sequence and is different from the first AID sequence, and wherein the second AMB sequence is a known sequence and is different from the first AMB sequence;
- and
- (c) one or more additional containers comprising reagents capable of conjugating the first oligonucleotide to a first affinity molecule and/or comprising reagents capable of conjugating the second oligonucleotide to a second affinity molecule;
- wherein the first affinity molecule is configured to bind to a first extracellular protein of a cell, and the second affinity molecule is configured to bind to a second extracellular protein of a cell, wherein the first extracellular protein is different from the second extracellular protein;
- and
- (d) a set of instructions describing how to conjugate the first oligonucleotide to the first affinity molecule and the second oligonucleotide to the second affinity molecule.
4 more elements
Dependencies are inferred from the claim text.