Multiplexed sensor for ultra-fast and low-cost covid-19 diagnosis and monitoring
AssigneeCalifornia Institute of Technology
InventorsWei Gao, Rebeca M. Torrente-Rodriguez, Heather Lukas
Life of this patent
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Abstract
A biosensor for the rapid, inexpensive, quantitative, and convenient detection of SARS-CoV-2 biomarkers, methods of manufacturing, and methods of using the same, to identify a patient's prognosis and past/present SARS-CoV-2 infection status, wherein the biosensor comprises a microfluidics layer, a multimodal sensing layer comprising two or more working electrodes, and a logic circuit that may include a processor and non-transitory memory with computer executable instructions embedded thereon.
Claims25
A biosensor for detecting SARS-CoV-2 biological markers, comprising:
- (a) a microfluidics layer;
- (b) a multimodal layer comprising:
- (i) a counter electrode;
- (ii) a reference electrode;
- (iii) a first working electrode comprising a first detection protein configured to capture a first SARS-CoV-2 biomarker;
- and
- (iv) a second working electrode comprising a second detection protein configured to capture a second SARS-CoV-2 biomarker;
- and
- (c) a logic circuit comprising a processor and a non-transitory memory with computer executable instructions embedded thereon;
- wherein the microfluidics layer comprises multiple microchannels transversely oriented to channel a biological sample from a first surface of the microfluidics layer to a second surface of the microfluidics layer, the biological sample comprising a first and/or second SARS-CoV-2 biomarker;
- the multimodal sensing layer is fluidically coupled to the second surface of the microfluidics layer to receive the biological sample from the microchannels;
- the electrodes configured to detect a measurement of an electrical property corresponding to the first and/or second SARS-CoV-2 biomarkers being present in the biological sample;
- and
- the logic circuit is electrically coupled to the electrodes and the computer executable instructions cause the processor to identify the electrical properties detected with the electrodes when the first and/or second SARS-CoV-2 biomarkers are present in the biological sample.
A method for detecting SARS-CoV-2 biological markers, comprising:
- (a) a biosensor comprising:
- (i) a microfluidics layer comprising a plurality of microchannels transversely oriented to channel a biological sample from a first surface of the microfluidics layer to a second surface of the microfluidics layer;
- (ii) a multimodal sensing layer fluidically coupled to the microfluidics layer comprising:
- (1) a counter electrode;
- (2) a reference electrode;
- (3) a first working electrode comprising a first detection protein configured to capture a first SARS-CoV-2 biomarker;
- and
- (4) a second working electrode comprising a second detection protein configured to capture a second SARS-CoV-2 biomarker;
- and
- (ii) a logic circuit, the method comprising:
- (b) receiving, on a first surface of the microfluidics layer, a biological sample comprising the first and/or second SARS-CoV-2 biomarkers, such that the biological sample can be channeled from the first surface of the microfluidics layer to the second surface of the microfluidics layer;
- (c) obtaining, with the electrodes, a measurement of an electrical property of the first and/or second SARS-CoV-2 biomarkers;
- and
- (d) generating, with the logic circuit, an indication that the first and/or second SARS-CoV-2 biomarkers is/are present in the biological sample based on the measurement of the electrical properties of the first and/or second SARS-CoV-2 biomarkers.
Dependencies are inferred from the claim text.