Method and kit for determining the tissue or cell origin of dna
AssigneesYissum Research Development Company of the Hebrew University of Jerusalem, Hadasit Medical Research Services and Development Ltd.
InventorsYuval DOR, Ruth SHEMER, Benjamin GLASER
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Abstract
A method of detecting death of a cell type or tissue in a subject is disclosed. The method comprises determining whether cell-free DNA comprised in a fluid sample of the subject is derived from the cell type or tissue, wherein the determining is effected by ascertaining the methylation status of at least four methylation sites on a continuous sequence of the cell-free DNA, the sequence comprising no more than 300 nucleotides, wherein a methylation status of each of the at least four methylation sites on the continuous sequence of the DNA characteristic of the cell type or tissue is indicative of death of the cell type or tissue. Kits for detecting cell death are also disclosed.
Claims20
A kit for identifying the source of DNA in a sample comprising, oligonucleotides which are capable of detecting the methylation status of at least four methylation sites in a nucleic acid sequence, said nucleic acid sequence being no longer than 300 base pairs and comprising at least four methylation sites which are differentially methylated in a first cell or tissue of interest with respect to a second cell or tissue which is non-identical to said first cell or tissue of interest.
A kit for identifying the source of DNA in a sample comprising at least oligonucleotides which are capable of amplifying a DNA haying a nucleic acid sequence no longer than 300 base pairs, wherein said nucleic acid sequence comprises at least four methylation sites which are differentially methylated in a first cell of interest with respect to a second cell which is non-identical to said first cell of interest.
Dependencies are inferred from the claim text.