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US 2022/0064719 A1Published application
Method of calibration of mfish using slides
AssigneeApplied Materials, Inc.
InventorsChloe Kim, Lu Yan
Life of this patent
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Abstract
A method of calibrating a fluorescence in-situ hybridization (FISH) system includes contacting a calibration slide with a plurality of probes, obtaining one or more images of the calibration slide; and calibrating the FISH system based on the one or more images. The calibration slide has a surface with a first plurality of beads. Each bead of the first plurality of beads has one or more binding domains. Each probe has a tag and a targeting domain, and the targeting domain binds a binding domain from the at least one binding domain.
Claims25
A method of calibrating a fluorescence in-situ hybridization (FISH) system, comprising:
- (a) contacting a calibration slide with a plurality of probes, wherein the calibration slide comprises a surface having a first plurality of beads, wherein each bead of the first plurality of beads has one or more binding domains, wherein each probe comprises a tag and a targeting domain, and wherein the targeting domain binds a binding domain from the at least one binding domain;
- (b) obtaining one or more images of the calibration slide;
- and
- (c) calibrating the FISH system based on the one or more images.
1 more element
Dependencies are inferred from the claim text.