Hybridization compositions and methods
AssigneeAgilent Technologies, Inc.
InventorsCharles M. Hansen, Steen Hauge Matthiesen, Kenneth H. Petersen, Tim Svenstrup Poulsen
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Abstract
The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, eliminate the use of, or reduce the dependence on formamide in hybridization. Compositions for use in the invention include an aqueous composition comprising at least one nucleic acid sequence and at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences.
Claims20
1-60. (canceled)
A hybridization composition comprising a mixture of at least one nucleic acid sequence, at least one polar aprotic solvent in an amount effective to denature double-stranded nucleotide sequences, an accelerating agent, and at least one additional component selected from the group consisting of:
- buffering agents, salts, chelating agents, detergents, and blocking agents,
- wherein less than 10% of the hybridization composition is formamide,
- wherein the polar aprotic solvent is not dimethyl sulfoxide (DMSO) and has lactone, sulfone, sulfite, and/or carbonate functional groups,
- wherein the at least one nucleic acid sequence is a FISH or CISH probe;
- wherein the at least one polar aprotic solvent comprises a polar aprotic solvent selected from the group consisting of:
- wherein the accelerating agent is a polymer.
Dependencies are inferred from the claim text.