Rapid reverse transcription quantitative polymerase chain reaction
AssigneeNorthwestern University
InventorsJennifer L. Reed, Sally M. McFall, Matthew A. Butzler
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Abstract
Provided herein are methods for rapid detection of RNA in a sample. The methods comprise providing a reaction mixture containing the sample, amplification reagents, and a polymerase enzyme having both RNA and DNA-dependent polymerase activity; reverse transcribing the RNA to DNA by incubating for a reverse transcription time of no longer than 5 minutes; and amplifying the DNA by performing a thermal cycling protocol comprising a plurality of amplification cycles, wherein each amplification cycle comprises at least a denaturation step and an annealing step.
Claims21
A method for detecting a target RNA in a sample comprising:
- a. Providing a reaction mixture containing the sample, amplification reagents, and a polymerase enzyme having both RNA and DNA-dependent polymerase activity;
- b. Reverse transcribing the RNA to DNA by incubating for a reverse transcription time of no longer than 5 minutes;
- c. Amplifying the DNA by performing a thermal cycling protocol comprising a plurality of amplification cycles, wherein each amplification cycle comprises at least a denaturation step and an annealing step.
Dependencies are inferred from the claim text.