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US 2022/0064704 A1Published application
METHODS OF PREPARING LARGE QUANTITIES OF SINGLE-STRANDED DNA (ssDNA)
AssigneeApplied Materials, Inc.
InventorsChung Pui Cheng, Tze Howe Charn
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Abstract
The disclosure relates to methods of preparing single-stranded DNA (ssDNA). The ssDNA can be used, for example, to prepare functionalized alignment beads as fiducial markers to improve image registration in fluorescence assays for the detection and quantitation of analytes in a sample.
Claims20
A method of generating single-stranded DNA (ssDNA), the method comprising:
- (a) providing a plurality of DNA oligonucleotides;
- (b) performing a polymerase chain reaction (PCR) amplification of the DNA oligonucleotides;
- (c) purifying the PCR products with magnetic beads;
- (d) performing in vitro transcription (IVT) reaction with the PCR products to generate intermediate RNA molecules;
- (e) performing reverse transcription (RT) of the intermediate RNA molecules to generate ssDNA;
- and
- (f) purifying the ssDNA with magnetic beads.
4 more elements
Dependencies are inferred from the claim text.