Multiplexed imaging reagent compositions and kits

A method for detecting multiple target analytes in a biological sample, the method comprising:

• (a) contacting a biological sample with a plurality of different types of probes, wherein each different type of probe comprises a capture moiety that selectively binds to a different target analyte in the sample, and an oligonucleotide having a sequence that is unique among other types of probes in the plurality of different types of probes;
• (b) binding an optical label to one of the different types of probes, wherein the optical label comprises an optical moiety linked to a labeling oligonucleotide, and wherein the labeling oligonucleotide comprises a sequence that hybridizes to the oligonucleotide sequence of the one type of probe;
• (c) contacting the sample with a composition comprising at least one blocking agent, wherein the at least one blocking agent comprises an oligonucleotide having a sequence that hybridizes to the oligonucleotide of another type of probe from among the different types of probes;
• and
• (d) obtaining an image of the sample comprising information corresponding to one or more locations of the one type of probe in the sample.