Target engagement assay for ras proteins
AssigneePromega Corporation
InventorsMatthew B. Robers, Joel R. Walker, James Vasta, Cesear Corona
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Abstract
Provided herein are systems, methods, and compounds for identifying RAS binding compounds using a RAS binding agent, which comprises a RAS binding moiety and a functional element. In some embodiments, the RAS binding agent binds to one site on the RAS protein (e.g., KRAS, HRAS, or NRAS), and can be used to detect RAS binding agents that bind to the same site as well as other sites.
Claims33
A method of identifying a RAS binding compound, the method comprising:
- (a) providing a sample comprising a RAS protein;
- and
- (b) contacting the sample with a RAS binding agent comprising a RAS binding moiety and a functional element, and a candidate RAS binding compound.
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A system comprising:
- (a) a target RAS protein;
- (b) a RAS binding agent comprising a RAS binding moiety and a functional element;
- and
- (c) a candidate RAS binding compound.
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A RAS binding agent comprising:
- (a) a RAS binding moiety;
- and
- (b) a functional element.
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Dependencies are inferred from the claim text.