Method for reducing misincorporation of non-canonical branched-chain amino acids

The method of any one of claim 5, wherein said microbial host cell does not express an endogenous polypeptide having said enzymatic activity. The method of any one of claims 1 to 6, wherein the polypeptide of interest is a therapeutic peptide or polypeptide.

A method for reducing misincorporation of at least one non-canonical branched-chain amino acid into a recombinant polypeptide of interest expressed in a microbial host cell, said method comprising

• (a) modulating an enzymatic activity selected from the group consisting of ketol-acid reductoisomerase (NADP(+)) activity (EC 1.1.1.86), acetohydroxyacid synthase activity (EC 2.2.1.6), aspartate kinase activity (EC 2.7.2.4), homoserine dehydrogenase activity (EC 1.1.1.3), and L-threonine dehydratase (EC 4.3.1.19) in the microbial host cell,
• (b) introducing a polynucleotide encoding the polypeptide of interest into said microbial host cell, and
• (c) expressing said polypeptide of interest in said microbial host cell.

Use of a polypeptide having an enzymatic activity selected from the group consisting of ketol-acid reductoisomerase (NADP(+)) activity (EC 1.1.1.86), acetohydroxyacid synthase activity (EC 2.2.1.6), aspartate kinase activity (EC 2.7.2.4), homoserine dehydrogenase activity (EC 1.1.1.3), and L-threonine dehydratase (EC 4.3.1.19), or of a polynucleotide encoding said polypeptide for reducing misincorporation of at least one non-canonical branched-chain amino acid into a recombinant polypeptide of interest produced in a microbial host cell.

Use of a microbial host cell for producing a recombinant polypeptide of interest, wherein the microbial host cell has been modified such that an enzymatic activity selected from the group consisting of ketol-acid reductoisomerase (NADP(+)) activity (EC 1.1.1.86), acetohydroxyacid synthase activity (EC 2.2.1.6), aspartate kinase activity (EC 2.7.2.4), homoserine dehydrogenase activity (EC 1.1.1.3), and L-threonine dehydratase (EC 4.3.1.19) is modulated in said microbial host cell as compared to the enzymatic activity in an unmodified microbial host cell.

A microbial host cell comprising

• (a) a recombinant polynucleotide encoding a polypeptide of interest, and
• (b) a recombinant polynucleotide encoding a polypeptide having an enzymatic activity
• selected from the group consisting of ketol-acid reductoisomerase (NADP(+)) activity (EC 1.1.1.86), acetohydroxyacid synthase activity (EC 2.2.1.6), aspartate kinase activity (EC 2.7.2.4), homoserine dehydrogenase activity (EC 1.1.1.3), and L-threonine dehydratase (EC 4.3.1.19).