CELL ENGINEERING USING RNAs
AssigneeBoehringer Ingelheim International GmbH
InventorsLore FLORIN, Hitto KAUFMANN, Angelika HAUSSER, Monilola OLAYIOYE, Michaela STROTBEK
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Abstract
The invention concerns the field of cell culture technology. It concerns RNA having a specific sequence, expression vectors encoding said RNA, production host cell lines comprising said RNA, and methods of producing recombinant biopharmaceutical products using engineered host cell with altered levels of said RNAs, such as small non-coding RNAs, preferably microRNAs (miRNAs). The invention also relates to engineered host cells with altered levels in one or more of said RNAs. Those cell lines have improved secretion and/or growth characteristics in comparison to control cell lines.
Claims29
(canceled)
A mammalian expression vector comprising a microRNA selected from the group consisting of:
- SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, and SEQ ID NO:20.
(canceled)
14-17. (canceled)
A mammalian cell comprising one or more microRNAs selected from one or more of the group consisting of:
- SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, and SEQ ID NO:20, wherein the cell is stably transfected with an expression vector encoding said one or more microRNA.
19-21. (canceled)
28-51. (canceled)
A CHO cell comprising one or more microRNAs selected from the group consisting of:
- miR-1287 (SEQ ID NO:6), miR-1978 (SEQ ID NO:11), and miR-557 (SEQ ID NO:16).
Dependencies are inferred from the claim text.