Use of substrate importers for the export of oligosaccharides
AssigneeZimitech
InventorsJames Cate, Kulika Chomvong, Oliver Kilian, Jingjing Liu, Jason Liu, Yong-Su Jin
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Abstract
Disclosed herein are genetically modified microorganisms and related methods for the enhanced export of oligosaccharides. The microorganisms described herein express major facility superfamily proteins such as CDT-1 which allows for the export of oligosaccharides. Variants of CDT-1 exhibit higher activity regarding oligosaccharide export. Means to export oligosaccharides into the growth medium are provided herein.
Claims36
A microorganism for enhanced production of a human milk oligosaccharide (HMO) comprising a heterologous CDT-1 transporter variant and at least one heterologous pathway gene for production of the HMO, wherein the microorganism is capable of producing and exporting the HMO.
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A microorganism for enhanced production of 2′-FL comprising a heterologous CDT-1 transporter or a variant thereof and at least one heterologous pathway gene for production of the HMO selected from the group consisting of GDP-mannose 4,6-dehydratase (GMD), a GDP-L-fucose synthase (GFS), and a fucosyl transferase (FT).
A microorganism for enhanced production of a human milk oligosaccharide (HMO) comprising (a) a heterologous CDT-1 transporter or a variant thereof;
- (b) at least one heterologous pathway gene for production of the HMO, wherein the microorganism is capable of producing and exporting the HMO and (c) a genetic modification that decreases the activity of SNF3 or RGT2 as compared to a parental microorganism without the genetic modification.
Dependencies are inferred from the claim text.