Biosynthetic methods and systems for producing monosaccharides
AssigneeCemvita Factory, Inc.
InventorTahereh Karimi
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Abstract
The present disclosure is related to biosynthetic methods of forming monosaccharides, and systems for generating the same. A benefit of the methods and systems disclosed herein can include the sustainable production of monosaccharides in an automated process. A benefit of the methods and systems herein can be the generation of monosaccharides from renewable source materials. An additional benefit of the methods and systems herein can include the use of abundant feedstocks, such as carbon dioxide, for the efficient generation of select monosaccharides for use as nutrients and for other useful applications. Another benefit of the methods and systems disclosed herein can include reduction of excess carbon dioxide from the environment.
Claims22
A method of forming a monosaccharide comprising:
- providing a hydrogen source containing hydrogen gas in an aqueous electrolyte solution;
- providing a carbon dioxide source;
- forming a reaction mixture by feeding the hydrogen source and the carbon dioxide source into a synthetic reaction vessel containing an aqueous reaction solution, wherein the aqueous reaction solution contains a plurality of photosynthetic enzymes, at least two cofactors including reduced nicotinamide adenine dinucleotide phosphate (NADPH) and adenine triphosphate (ATP), and at least one substrate;
- and
- forming an amount of the monosaccharide in the synthetic reaction vessel by reacting the hydrogen source, the carbon dioxide source, and the at least one substrate in contact with the plurality of photosynthetic enzymes and the at least two cofactors.
A synthetic system for generating a monosaccharide from carbon dioxide and water comprising:
- a hydrolysis electrochemical reactor including a power source;
- a carbon dioxide source;
- a monosaccharide generator vessel containing a hydrogen fluid flow path in contact with the hydrolysis electrochemical reactor cell, a carbon dioxide fluid flow path in contact with the carbon dioxide source, and an aqueous reaction solution containing a plurality of photosynthetic enzymes, at least two cofactors including reduced nicotinamide adenine dinucleotide phosphate (NADPH) and adenine triphosphate (ATP), and at least one substrate.
A method of forming a monosaccharide comprising:
- providing a hydrogen source containing hydrogen gas in an aqueous electrolyte solution;
- providing a carbon dioxide source;
- forming a reaction mixture by feeding the hydrogen source and the carbon dioxide source into a cellular reaction vessel containing a Cyanobacteria sp. in an aqueous reaction solution, at least two cofactors including reduced nicotinamide adenine dinucleotide phosphate (NADPH) and adenine triphosphate (ATP), and at least one substrate, wherein the Cyanobacteria sp. expresses a plurality of photosynthetic enzymes;
- and
- forming an amount of the monosaccharide in the synthetic reaction vessel by reacting the hydrogen source, the carbon dioxide source, and the at least one substrate in contact with the plurality of photosynthetic enzymes and the at least two cofactors.
Dependencies are inferred from the claim text.