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US 2022/0064678 A1Published application
Method for production of vitamin k using biofilm reactors
AssigneesThe Penn State Research Foundation, The University of Waikato
InventorsAli DEMIRCI, Ehsan MAHDINIA, Aydin BERENJIAN
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Abstract
Provided are improved methods for Vitamin K, including but not necessarily limited to MK-7 production through bacterial fermentation using biofilm reactors. Fed-batch addition of carbon sources, such as glucose, are used as the base media in biofilm reactors. Fed-batch strategies are shown to be significantly effective in glucose-based medium, increasing the end-product concentrations to more than 2-fold higher than the level produced in suspended-cell bioreactors.
Claims26
A method for producing Vitamin K, the method comprising:
- i) introducing into a biofilm reactor a first glucose or glycerol containing bacteria culture media, wherein a biofilm comprising bacteria that are capable of producing the Vitamin K forms on a surface in the biofilm reactor, wherein optionally the first glucose or glycerol containing bacteria culture media is replaced one or more times during formation of the biofilm;
- ii) introducing a second glycerol or glucose containing bacteria culture media into the biofilm reactor, agitating the bacteria culture medium;
- and
- iii) separating the Vitamin K from the biofilm reactor.
1 more element
A system comprising:
- a biofilm reactor and a plurality of plastic composite supports (PCS), the plurality of PCS supports comprising a biofilm comprising bacteria that produce Vitamin K, the system further comprising a bacterial culture media comprising glucose, glycerin, or a combination thereof, wherein the bacterial culture media comprises at least 24-30 mg/L of the Vitamin K.
Dependencies are inferred from the claim text.