Methods and microorganisms for making 1,4-butanediol and derivatives thereof from c1 carbons
AssigneePrecigen, Inc.
InventorsXinhua ZHAO, Tina HUYNH, Jeffrey ORTH, Lily Yuin CHAO, James KEALEY
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Abstract
Genetically modified microorganisms that have the ability to convert carbon substrates into chemical products such as 1,4-BDO are disclosed. For example, genetically modified methanotrophs that are capable of generating 1,4-BDO at high titers from a methane source are disclosed. Methods of making these genetically modified microorganisms and methods of using them are also disclosed.
Claims35
A genetically modified microorganism capable of converting a single carbon-containing hydrocarbon molecule to a multicarbon product, the microorganism comprising a heterologous gene encoding pyruvate dehydrogenase, citrate synthase, aconitate hydratase 1, isocitrate dehydrogease, α-ketoglutarate decarboxylase, 4-hyrobutyrate dehydrogenase, 4-hydroxybutyryl-CoA transferase, aldehyde dehydrogenase, alcohol dehydrogenase, succinyl-CoA synthetase, or CoA-dependent succinate semialdehyde dehydrogenase.
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A vector encoding at least two of the following:
- pyruvate dehydrogenase;
- citrate synthase;
- aconitate hydratase 1;
- isocitrate dehydrogenase;
- α-ketoglutarate decarboxylase;
- succinyl-CoA synthetase;
- CoA-dependent succinate semialdehyde dehydrogenase;
- 4-hyrobutyrate dehydrogenase;
- 4 hydroxybutyryl CoA transferase;
- aldehyde dehydrogenase;
- and alcohol dehydrogenase.
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Dependencies are inferred from the claim text.