US 2022/0064666 A1 · SYNTHETIC MAMMALIAN SIGNALING CIRCUITS FOR ROBUST CELL POPULATION CONTROL

Abstract

Disclosed herein include circuits, compositions, nucleic acids, populations, systems, and methods enabling cells to sense, control, and/or respond to their own population size. In some embodiments, an orthogonal communication channel allows specific communication between engineered cells. Also described herein, in some embodiments, is an evolutionarily robust ‘paradoxical’ regulatory circuit architecture in which orthogonal signals both stimulate and inhibit net cell growth at different signal concentrations. In some embodiments, engineered cells autonomously reach designed densities and/or activate therapeutic or safety programs at specific density thresholds. Methods of treatment are also provided in some embodiments.

Claims35

Dependent claims

A nucleic acid composition, comprising:

a first polynucleotide encoding a first synthase, wherein the first synthase is capable of catalyzing the synthesis of an orthogonal signal from a first precursor molecule;
a second polynucleotide encoding a second synthase, wherein the second synthase is capable of catalyzing the synthesis of a first precursor molecule from a second precursor molecule;
and/or
a third polynucleotide encoding a transporter capable of transporting an orthogonal signal, a first precursor molecule, and/or a second precursor molecule across a cell membrane.

1 more element

Dependencies are inferred from the claim text.

Synthetic mammalian signaling circuits for robust cell population control

Life of this patent

Synthetic mammalian signaling circuits for robust cell population control

Life of this patent

Abstract

Claims35