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AssigneeSeminis Vegetable Seeds, Inc.
InventorsJohannes Hendrikus Maria Barten, Susana Garcia-Andres, Benjamin C. Hunter, Francisco Monci Marin, Maria Belen Salleres Neira
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Tomato plants exhibiting resistance to tomato chlorosis virus and Fusarium oxysporum f. sp. lycopersici race 2 are provided, together with methods of producing, identifying, or selecting plants or germplasm with a tomato chlorosis virus and Fusarium oxysporum f. sp. lycopersici race 2 resistance phenotype and lacking an undesirable cold sensitivity trait. Such plants include tomato plants comprising recombinant genomic regions conferring disease resistance. Compositions, including novel polymorphic markers for detecting plants comprising introgressed disease resistance alleles, are further provided.
A Solanum lycopersicum plant comprising a recombinant chromosomal segment on chromosome 11, wherein said chromosomal segment comprises a tomato chlorosis virus (ToCV) resistance allele from Solanum chilense that confers to said plant an increased resistance to ToCV compared to a plant not comprising said allele, and wherein:
A recombinant DNA segment comprising a tomato chlorosis virus (ToCV) resistance allele from Solanum chilense that confers increased resistance to ToCV and lacks an allele genetically linked thereto that confers cold sensitivity when present, wherein said DNA segment comprises a Fusarium oxysporum f. sp. lycopersici (Fol) race 2 resistance allele from Solanum pimpinellifolium that confers increased resistance to Fol race 2, and wherein said Fol race 2 resistance allele in cis linkage with said ToCV resistance allele.
A method of producing a tomato plant with improved tomato chlorosis virus (ToCV) resistance, comprising introgressing into said plant at least one ToCV resistance allele from Solanum chilense within a recombinant chromosomal segment flanked in the genome of said plant by marker locus M1 (SEQ ID NO:6) and marker locus M3 (SEQ ID NO:16) on chromosome 11, wherein said introgressed ToCV resistance allele confers to said plant increased resistance to ToCV compared to a plant not comprising said allele, and wherein said recombinant chromosomal segment lacks a deleterious allele genetically linked thereto that confers a cold sensitivity phenotype when present, and wherein said introgressing comprises marker-assisted selection.
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