Engineered nucleases to generate mutations in plants
AssigneeCargill, Incorporated
InventorMeridith KAMMERZELL
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Abstract
Methods are provided to mutate, in a targeted manner, the genome of a plant cell using a double stranded DNA break inducing enzyme. Also provided are plants, in particular Brassica plants that yield seeds producing oils having a reduced total saturated fatty acid content, and method for making such plants.
Claims21
A method to produce a Brassica plant with reduced saturated fatty acids comprising:
- a) inducing a double stranded DNA break at a target site, said double stranded break being induced by the introduction to a plant cell of a double stranded DNA break inducing (DSBI) enzyme which recognizes a recognition sequence in the vicinity of or at said target site in sec62, KCR2, DGAT1, FAX1, MIR394 and/or HAD sequence(s);
- and
- b) selecting a plant cell wherein said double stranded DNA break has been repaired resulting in a mutation in the genome at said target site, wherein said mutation is a substitution of at least one nucleotide, a deletion of at least one nucleotide, an insertion of at least one nucleotide or any combination thereof;
- and c) regenerating said plant cell(s) of b) into a plant;
- wherein said plant yields a reduction of stearic acid (18:0) of about 6% to about 27% as compared to a non-mutated plant of identical genetic background.
A mutated Brassica plant comprising a deletion mutation in at least one allele of sec62, KCR2, DGAT1, FAX1, MIR394 and/or HAD, wherein said deletion mutation results in a reduction of stearic acid (18:0) production of about 6% to about 27% in said plant as compared to a control plant of identical genetic background that does not have said deletion mutation.
A mutated Brassica plant comprising an insertion mutation in at least one allele of sec62, KCR2, DGAT1, FAX1, MIR394 and/or HAD, wherein said insertion mutation results in a reduction of stearic acid (18:0) production of about 6% to about 27% in said plant as compared to a control plant of identical genetic background that does not have said insertion mutation.
21-28. (canceled)
Dependencies are inferred from the claim text.