Novel x-aptamers for the use in detection of snap25
AssigneeGalderma Holding SA
InventorsEmilia LEKHOLM, Erica FORSBERG, Kimia HOSSEINI, Robert FREDRIKSSON, Anh-Tri DO
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Abstract
Described are methods for utilizing X-aptamers for detecting human SNAP25 and fragments thereof, and compositions comprising the X-aptamers.
Claims20
A synthetic oligonucleotide sequence selected from the group consisting of SEQ ID NOs:1-96.
A composition comprising a synthetic oligonucleotide sequence selected from the group consisting of SEQ ID NOs:1-96.
A kit comprising a synthetic oligonucleotide sequence selected from the group consisting of SEQ ID NOs:1-96.
A method of isolating SNAP25, or a fragment thereof, the method comprising:
- (a) administering a composition comprising SNAP25, or a fragment thereof, to a composition comprising one or more synthetic oligonucleotide sequences selected from the group consisting of SEQ ID NOs:1-96, wherein the synthetic oligonucleotide sequences are immobilized;
- (b) washing impurities from the SNAP25 or the fragment thereof bound to the immobilized synthetic oligonucleotide;
- and
- (c) eluting the SNAP25 or the fragment thereof from the immobilized synthetic oligonucleotide sequences.
A method of binding SNAP25, or a fragment thereof, with one or more X-aptamers, the method comprising administering a composition comprising one or more synthetic X-aptamers selected from the group consisting of SEQ ID NOs:1-96 to a composition comprising the SNAP25 or the fragment thereof, and wherein the one or more synthetic X-aptamers bind to the SNAP25 or the fragment thereof.
Dependencies are inferred from the claim text.