Inhibition of unintended mutations in gene editing
AssigneeShanghaitech University
InventorsJia Chen, Bei Yang, Li Yang, Xingxu Huang, Lijie Wang
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Abstract
Provided are fusion proteins and related molecules useful for conducting base editing with reduced or no off-target mutations. The fusion proteins may include a first fragment comprising a nucleobase deaminase or a catalytic domain thereof, a second fragment comprising a nucleobase deaminase inhibitory domain, and a protease cleavage site between the first fragment and the second fragment. Also provided are improved prime editing systems, including prime editing guide RNA with improved stability.
Claims22
A fusion protein comprising:
- a first fragment comprising a nucleobase deaminase or a catalytic domain thereof,
- a second fragment comprising a nucleobase deaminase inhibitor, and
- a protease cleavage site between the first fragment and the second fragment.
12-13. (canceled)
19-36. (canceled)
A fusion protein comprising:
- a first fragment comprising first nucleobase deaminase or a catalytic domain thereof, and
- a second fragment comprising an inhibitory domain of a second nucleobase deaminase,
- wherein the first nucleobase deaminase is same as or different from the second nucleobase deaminase.
A fusion protein comprising a first fragment comprising:
- a nucleobase deaminase or a catalytic domain thereof,
- a nucleobase deaminase inhibitor,
- a first RNA recognition peptide, and
- a TEV protease cleavage site between the nucleobase deaminase or a catalytic domain thereof and the nucleobase deaminase inhibitor.
40-64. (canceled)
Dependencies are inferred from the claim text.