Polypeptide Variants
AssigneeNovozymes A/S
InventorsLars Henrik Oestergaard, Gernot J. Abel, Dorte Marie Koefoed Klitgaard, Annette Helle Johansen
Life of this patent
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Abstract
The present invention relates to polypeptide variants and methods for obtaining variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims16
A DNase variant, comprising an substitution at one or more positions corresponding to positions 4, 17, 19, 36, 38, 40, 41, 45, 51, 53, 54, 55, 57, 64, 66, 67, 68, 69, 70, 71, 72, 74, 75, 77, 82, 83, 84, 85, 86, 88, 91, 99, 101, 105, 106, 115, 116, 135, 136, 138, 139, 140, 141, 151, 152, 153, 154, 162, 163, 164, 166, 168, 169, 173, 182, 183, 184, 185, 186, 189, 189, 212, and 215 of SEQ ID NO:
- 1, wherein the variant has a sequence identity to the polypeptide shown in SEQ ID NO:
- 1 of at least 80%, wherein the variant has DNase activity and wherein the variant has improved stability as compared to the SEQ ID NO:
- 1.
(canceled)
A method for obtaining a DNase variant, comprising
- (a) introducing into a parent DNase an alteration at one or more positions corresponding to positions 4, 17, 19, 36, 38, 40, 41, 45, 51, 53, 55, 57, 64, 66, 67, 68, 69, 70, 71, 72, 74, 75, 77, 82, 83, 84, 85, 86, 88, 91, 99, 101, 105, 106, 115, 116, 135, 136, 138, 139, 140, 141, 151, 152, 153, 154, 162, 163, 164, 166, 168, 169, 173, 182, 183, 184, 185, 186, 189, 189, 212, or 215 of SEQ ID NO:
- 1, wherein the variant has an amino acid sequence which is at least 80% identical to SEQ ID NO:
- 1, and
- (b) recovering the variant.
Dependencies are inferred from the claim text.