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AssigneeJiangsu Shiyutian Biotechnology Co., Ltd.
InventorYujie Chen
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The present invention discloses a recombinant Bacillus subtilis strain for producing UDP-glycosyltransferase and a recombination method therefor. The recombination method includes the following steps: chemically synthesizing a UDP-glycosyltransferase gene UGT and linking the UGT with a vector pUC57 to obtain pUC57-UGT, and cloning various promoters; linking the obtained pUC57-UGT and each of the promoters to an expression vector to obtain recombinant plasmids; transforming the obtained recombinant plasmids to host strains, respectively, to obtain recombinant plasmids of the host strains; respectively transforming the obtained recombinant plasmids of the host strains to Bacillus subtilis to obtain recombinant strains; and screening out recombinant Bacillus subtilis strain highly expressing UDP-glycosyltransferase from the obtained recombinant strains.
A recombinant Bacillus subtilis strain for producing UDP-glycosyltransferase, wherein the recombinant strain is obtained by expressing a UDP-glycosyltransferase gene in a microorganism, wherein the UDP-glycosyltransferase gene is linked to an expression vector together with each of various promoters, and then transformed to a host strain of Bacillus subtilis to construct the recombinant strain of the UDP-glycosyltransferase gene.
A recombination method for preparing a recombinant Bacillus subtilis strain for producing UDP-glycosyltransferase, comprising the following steps:
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