Novel acetyl-transferases

A retinol-acetylating enzyme, preferably fungal enzyme, comprising one or more amino acid substitution(s) in a sequence with at least about 20%, such as 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 92, 95, 97, 98, 99% or up to 100% identity to SEQ ID NO:1, wherein the one or more amino acid substitution(s) are located at position(s) corresponding to amino acid residue(s) selected from the group consisting of position 68, 69, 72, 73, 171, 174, 176, 178, 291, 292, 294, 301, 307, 308, 311, 312, 320, 322, 334, 362, 405, 407, 409, 480, 483, 484, 490, 492, 520, 521, 522, 524, 525, 526 and combinations thereof, in the polypeptide according to SEQ ID NO:1, and wherein the one or more substitute residue(s) is/are selected from the group consisting of substitute residue corresponding to position 68 is threonine, substitute residue corresponding to position 69 is asparagine or serine or adenine, substitute residue corresponding to position 72 is asparagine or lysine, substitute residue corresponding to position 73 is leucine, substitute residue corresponding to position 171 being lysine or asparagine, substitute residue corresponding to position 172 is glycine, substitute residue corresponding to position 174 is isoleucine, substitute residue corresponding to position 176 is alanine or glycine, substitute residue corresponding to position 178 is valine, substitute residue corresponding to position 291 is serine or glycine, substitute residue corresponding to position 292 is alanine or serine or asparagine, substitute residue corresponding to position 294 is leucine or valine, substitute residue corresponding to position 301 is phenylalanine, substitute residue corresponding to position 307 is isoleucine, substitute residue corresponding to position 308 is valine, substitute residue corresponding to position 311 is methionine or isoleucine, substitute residue corresponding to position 312 is alanine, substitute residue corresponding to position 320 is asparagine, substitute residue corresponding to position 322 is valine or phenylalanine, substitute residue corresponding to position 334 is leucine, substitute residue corresponding to position 362 is alanine, substitute residue corresponding to position 405 is alanine, substitute residue corresponding to position 407 is isoleucine, substitute residue corresponding to position 409 is alanine, substitute residue corresponding to position 480 is glutamic acid or lysine or methionine or phenylalanine or glutamine, substitute residue corresponding to position 483 is valine, substitute residue corresponding to position 484 is leucine, substitute residue corresponding to position 490 is isoleucine, substitute residue corresponding to position 492 is valine, substitute residue corresponding to position 520 is alanine, substitute residue corresponding to position 521 is alanine or valine, substitute residue corresponding to position 522 is serine or threonine, substitute residue corresponding to position 524 is asparagine or serine, substitute residue corresponding to position 525 is valine or isoleucine or arginine, substitute residue corresponding to position 526 is serine, and combinations thereof in the polypeptide according to SEQ ID NO:1.