A physiological biomimetic culture system for heart slices
AssigneeUniversity of Louisville Research Foundation, Inc.
InventorTamer MOHAMED
Life of this patent
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Abstract
A method for culturing heart tissue by culturing a slice of the heart tissue in a culture medium, wherein the culture medium comprises fetal bovine serum (FBS), vascular endothelial cell growth factor (VEGF), and fibroblast growth factor (FGF); and applying electrical stimulation to the slice while the heart slice is in culture is provided. Methods of screening candidate therapeutic agents for therapeutic effect or cardiotoxicity using the culture system are also provided.
Claims17
A method for culturing heart tissue, comprising:
- culturing a slice of the heart tissue in a culture medium, wherein the culture medium comprises fetal bovine serum (FBS), vascular endothelial cell growth factor (VEGF), and fibroblast growth factor (FGF);
- and
- applying electrical stimulation to the slice while the heart slice is in culture.
A method for screening candidate therapeutic agents for therapeutic effect or cardiotoxicity, comprising:
- culturing a slice of the heart tissue in a culture medium, wherein the culture medium comprises fetal bovine serum (FBS), vascular endothelial cell growth factor (VEGF), and fibroblast growth factor (FGF);
- applying electrical stimulation to the slice while the heart slice is in culture;
- contacting the slice with a candidate therapeutic agent;
- and
- measuring one or more factors within the heart tissue that are indicative of the therapeutic effect or cardiotoxicity of the candidate drug.
Dependencies are inferred from the claim text.