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US 2022/0064597 A1Published application
Generation of organoid-primed T (opT) cells with memory phenotype
OwnerManuel Hidalgo, Senthil K. Muthuswamyinventors
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Abstract
A cell culture platform that uses a combination of sophisticated tissue engineering technologies to co-culture patient-derived tumor cells and the patient's own immune cells and create the conditions for expansion of tumor targeting T cells in culture. The platform can be used, e.g., for personalized medicine.
Claims14
A method of preparing a co-culture, the method comprising:
- obtaining cells from a tumor in a first subject, and preparing a tumor organoid from the cells;
- obtaining lymphocytes from the first subject or a second subject, and suspending the lymphocytes in media comprising one, two, or all three of IL-2, IL-15, and IL-21;
- and
- maintaining a co-culture comprising the tumor organoid in the presence of the lymphocytes in media comprising IL-2, IL-15, IL-21 and polyinosinic:polycytidylic acid.
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Dependencies are inferred from the claim text.