System and methods for sub-population identification
AssigneeABS Global, Inc.
InventorsJim HARRINGTON, Dan ANTONUK, Fred SAVAGE, David APPLEYARD, Matthew EBERSOLE
Life of this patent
Estimating the term…
Abstract
Systems and methods are provided for identification and discrimination of subpopulations within a mixture of particles. The systems and methods implement continuous calibration of the classification of particles within the mixture to provide consistency in operation and to reduce inter- and intra-batch processing variation. The systems and methods produce advantageously sorted particle products.
Claims24
A method for identifying subpopulations within a mixture of particles, comprising:
- measuring a characteristic of a first set of particles in a mixture of particles having two or more sub-populations, each sub-population being distinguishable by the characteristic;
- distinguishing the values of the measured characteristics for each of the sub-populations in the mixture;
- assigning a critical range of values to each of the sub-populations based on the distinguishing;
- subsequent to the assigning a critical range of values, measuring the characteristics of a second set of particles in the mixture of particles;
- subsequent to measuring the characteristics of a second set of particles in the mixture of particles, distinguishing the values of the measured characteristics for each of the sub-populations in the second set of particles;
- updating the critical range of values based on the distinguishing of the values of the measured characteristics for each of the sub-populations in the second set of particles;
- and
- assigning a sub-population classification to particles in the mixture based on the updated critical range of values.
(canceled)
A method for identifying multiple sets of particles within a mixture of sets of particles, each sub-population having a characteristic different from other sub-populations, comprising:
- providing the mixture of particles;
- detecting a characteristic of a first set of multiple particles within the mixture to produce a signal indicative of the characteristic for the first set of particles;
- generating event data for the signal indicative of the characteristic for said first set of particles;
- compiling the event data for the first set of particles;
- generating a distribution of event data for the first set of particles;
- calculating at least one discrimination value that defines a sub-population of particles based on the distribution of event data for the first set of particles;
- classifying particles based on the discrimination value that defines the subpopulation.
(canceled)
(canceled)
A system for producing a sexed sperm sample, comprising:
- a sample delivery apparatus for delivery of a mixture of sperm cells.
- a flow cytometry apparatus,
- a control configured to:
- distinguish the values of measured characteristics for each sub-population in the mixture of sperm cells,
- assign a critical range of values to each of the sub-populations based on the distinguishing,
- updating the critical range of values based on the distinguishing of the values of the measured characteristics for sub-populations in a second set of particles, and
- assigning a sub-population classification to particles in the mixture based on the updated critical range of values;
- and
- a sorting apparatus.
(canceled)
Dependencies are inferred from the claim text.