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US 2022/0064590 A1Published application
Microbes and methods for producing the same
AssigneeBayer CropScience AG
InventorsVarghese P. THOMAS, Benjamin L. GOLOMB, Damian CURTIS
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Abstract
The disclosure is generally directed to methods for screening, identifying, and producing microorganisms capable of imparting beneficial properties to plants. In some aspects, improved plant-associated soil microorganisms are generated by experimental evolution using a plant root exudate or root exudate compound.
Claims22
A method for producing plant-associated soil microbial (PASM) cells, comprising:
- (a) growing a genetically-uniform population of PASM cells in or on a first medium comprising a soil inhibitor;
- (b) harvesting at least some of the resulting PASM cells and growing the harvested PASM cells in or on a second medium comprising the soil inhibitor;
- (c) repeating step (b) at least one time;
- and
- (d) selecting at least one PASM cell that is different compared to the genetically-uniform population.
2 more elements
A method for producing PASM cells, comprising:
- (i) growing a genetically-uniform population of PASM cells in a chemostat in a medium comprising a soil inhibitor;
- and
- (ii) selecting at least one PASM cell that is different compared to the genetically-uniform population.
Dependencies are inferred from the claim text.