Delivery of biomolecules to immune cells
AssigneesMassachusetts Institute of Technology, President and Fellows of Harvard College
InventorsArmon R. Sharei, Shirley Mao, George Hartoularos, Sophia Liu, Megan Heimann, Pamela Basto, Gregory Szeto, Siddharth Jhunjhunwala, Darrell J. Irvine, Robert S. Langer, Klavs F. Jensen, Ulrich H. Von Andrian
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Abstract
A method and device for preferentially delivering a compound such as an antigen to the cytosol of an immune cell. The method comprises passing a cell suspension comprising the target immune cell through a microfluidic device and contacting the suspension with the compound(s) or payload to be delivered.
Claims29
1-113. (canceled)
A method of treating a patient by introducing modified immune cells to the patient, wherein the immune cells are modified by delivering a compound to the cytosol of the immune cell by passing a cell suspension comprising said immune cell through a microfluidic device and contacting said cell suspension with said compound, wherein said microfluidic device comprises a constriction having a diameter of 2 μm to 10 μm.
119-122. (canceled)
Dependencies are inferred from the claim text.