Functionalized substrate to manipulate cell function and differentiation
AssigneeKoninklijke Philips N.V.
InventorsAnja VAN DE STOLPE, LUCAS JOHANNES ANNA MARIA BECKERS
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Abstract
The invention relates to a scaffold for steering cells into a predetermined direction of cell functionality, preferably a cell differentiation scaffold for steering cells into a predetermined direction of cell differentiation. The scaffold comprises a polydimethylsiloxane (PDMS)-, or rubber-, or silicone-based polymeric surface, and one or more cell functionality-inducing stimuli, preferably one or more cell differentiation-inducing stimuli, coupled to the polymeric surface.
Claims19
A substrate, for steering of cells into a predetermined direction of cell functionality, the steering of cells consisting of steering cells into a predetermined direction of cell differentiation, the substrate comprising:
- an elastomer material having coupled thereto one or more cell functionality-inducing stimuli wherein the cell functionality-inducing stimuli comprise cell differentiation-inducing stimuli.
An In vitro method of producing a cell culture, tissue or organ, the method comprising:
- (a) determining first culture conditions that control activity of at least one first signaling pathway of cells to be cultivated, the activity of the at least one first signaling pathway being a selected activity to steer the cells into a first predetermined direction of cell functionality;
- and
- (b) cultivating cells in a culture unit under the first culture conditions to obtain a cell culture.
Dependencies are inferred from the claim text.