Method for treating glioblastoma or glioma with anitbody constructs egfrviii and cd3

A nucleic acid encoding a polypeptide comprising:

• (a) a first binding domain that binds to human and macaque epidermal growth factor receptor VIII (EGFRVIII), and comprises:
• a heavy chain variable region (VH) comprising the amino acid sequence of SEQ ID NO:
• 157, and a light chain variable region (VL) comprising the amino acid sequence of SEQ ID NO:
• 158;
• and
• (b) a second binding domain that binds to human CD3, and comprises:
• a VL region comprising:
• a CDR-L1 comprising the amino acid of SEQ ID NO:
• 92, a CDR-L2 comprising the amino acid of SEQ ID NO:
• 93, and a CDR-L3 comprising the amino acid of SEQ ID NO:
• 94;
• and a VH region comprising:
• a CDR-H1 comprising the amino acid of SEQ ID NO:
• 95, a CDR-H2 comprising the amino acid of SEQ ID NO:
• 96, and a CDR-H3 comprising the amino acid of SEQ ID NO:
• 97.