US 2022/0064296 A1 · BISPECIFIC T CELL ACTIVATING ANTIGEN BINDING MOLECULES

Abstract

The present invention generally relates to novel bispecific antigen binding molecules for T cell activation and re-direction to specific target cells. In addition, the present invention relates to polynucleotides encoding such bispecific antigen binding molecules, and vectors and host cells comprising such polynucleotides. The invention further relates to methods for producing the bispecific antigen binding molecules of the invention, and to methods of using these bispecific antigen binding molecules in the treatment of disease.

Claims79

Dependent claims

A T cell activating bispecific antigen binding molecule comprising

(i) a first antigen binding moiety which is a Fab molecule capable of specific binding to CD3, comprising at least one heavy chain complementarity determining region (CDR) selected from the group consisting of SEQ ID NO:
4, SEQ ID NO:
5 and SEQ ID NO:
6 and at least one light chain CDR selected from the group of SEQ ID NO:
8, SEQ ID NO:
9 and SEQ ID NO:
10;
(ii) a second antigen binding moiety which is a Fab molecule capable of specific binding to a target cell antigen.

5 more elements

A T cell activating bispecific antigen binding molecule comprising

(i) a first antigen binding moiety which is a Fab molecule capable of specific binding to CD3, comprising the heavy chain complementarity determining region (CDR) 1 of SEQ ID NO:
4, the heavy chain CDR 2 of SEQ ID NO:
5, the heavy chain CDR 3 of SEQ ID NO:
6, the light chain CDR 1 of SEQ ID NO:
8, the light chain CDR 2 of SEQ ID NO:
9 and the light chain CDR 3 of SEQ ID NO:
10, wherein the first antigen binding moiety is a crossover Fab molecule wherein either the variable or the constant regions, particularly the constant regions, of the Fab light chain and the Fab heavy chain are exchanged;
(ii) a second and a third antigen binding moiety each of which is a Fab molecule capable of specific binding to CEA comprising the heavy chain CDR 1 of SEQ ID NO:
24, the heavy chain CDR 2 of SEQ ID NO:
25, the heavy chain CDR 3 of SEQ ID NO:
26, the light chain CDR 1 of SEQ ID NO:
28, the light chain CDR 2 of SEQ ID NO:
29 and the light chain CDR3 of SEQ ID NO:
30.

11 more elements

A T cell activating bispecific antigen binding molecule comprising

(i) a first antigen binding moiety which is a Fab molecule capable of specific binding to CD3, comprising the heavy chain complementarity determining region (CDR) 1 of SEQ ID NO:
4, the heavy chain CDR 2 of SEQ ID NO:
5, the heavy chain CDR 3 of SEQ ID NO:
6, the light chain CDR 1 of SEQ ID NO:
8, the light chain CDR 2 of SEQ ID NO:
9 and the light chain CDR 3 of SEQ ID NO:
10, wherein the first antigen binding moiety is a crossover Fab molecule wherein either the variable or the constant regions, particularly the constant regions, of the Fab light chain and the Fab heavy chain are exchanged;
(ii) a second and a third antigen binding moiety each of which is a Fab molecule capable of specific binding to MCSP comprising the heavy chain CDR 1 of SEQ ID NO:
14, the heavy chain CDR 2 of SEQ ID NO:
15, the heavy chain CDR 3 of SEQ ID NO:
16, the light chain CDR 1 of SEQ ID NO:
18, the light chain CDR 2 of SEQ ID NO:
19 and the light chain CDR3 of SEQ ID NO:
20.

11 more elements

Dependencies are inferred from the claim text.

Bispecific t cell activating antigen binding molecules

Life of this patent

Bispecific t cell activating antigen binding molecules

Life of this patent

Abstract

Claims79