Fusion proteins comprising pdgf and vegf binding portions and methods of using thereof
AssigneeGenzyme Corporation
InventorsPeter PECHAN, Jeffery ARDINGER, Hillard RUBIN, Samuel WADSWORTH, Abraham SCARIA
Life of this patent
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Abstract
The present provides fusion proteins comprising PDGF and VEGF binding portions, and recombinant viral particles encoding the fusion proteins. Compositions comprising the fusion proteins and viral particles as well as methods of using the same are also provided.
Claims22
1-50. (canceled)
A method of delivering a viral vector to a subject, comprising administering an rAAV particle comprising a nucleic acid encoding a fusion protein;
- wherein the fusion protein comprises (a) an extracellular portion of a PDGF receptor comprising at least Ig-like domains D1-D3 of a PDGF receptor beta (PDGFRβ), (b) an extracellular portion of a VEGF receptor 1 (Flt-1) comprising at least an Ig-like domain D2, and (c) a multimerization domain, wherein the fusion proteins bind to a PDGF and a VEGF, and the fusion protein is arranged from N-terminal to C-terminus in the following order:
- (a), (b), and (c);
- wherein the fusion protein does not comprise an Ig-like domain D3 of a VEGFR2.
A method of delivering a viral vector to a subject, comprising administering by intravitreal injection an rAAV particle comprising a nucleic acid encoding a fusion protein;
- wherein the fusion protein comprises (a) an extracellular portion of a PDGF receptor comprising at least Ig-like domains D1-D3 of a PDGF receptor beta (PDGFRβ), (b) an extracellular portion of a VEGF receptor 1 (Flt-1) comprising at least an Ig-like domain D2, and (c) a multimerization domain, wherein the fusion proteins bind to a PDGF and a VEGF, and the fusion protein is arranged from N-terminal to C-terminus in the following order:
- (a), (b), and (c);
- wherein the fusion protein further comprises at least one of a linker peptide between the extracellular portion of the PDGF receptor and the extracellular portion of the VEGF receptor and a peptide linker between the extracellular portion of the VEGF receptor and the multimerization domain;
- wherein the fusion protein does not comprise an Ig-like domain D3 of a VEGFR2.
Dependencies are inferred from the claim text.