Binding Domain Mapping and Compounds, Compositions, Complexes, Methods, and Kits Related Thereto
AssigneeGeorge Mason Research Foundation, Inc.
InventorsLance Liotta, Alessandra Luchini Kunkel, Amanda Still, Mikell Paige
Life of this patent
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Abstract
The present disclosure relates to compounds, complexes, compositions, kits and methods for determining interacting and/or binding sites between e.g., proteins, proteins and nucleic acids, proteins and small molecules, or intrachain protein domains. The disclosure provides rapid and direct positive identification of the contact interface region between such molecules, and can be applied to individual interacting pairs, as well as large-scale or global interactions.
Claims33
A compound having Formula (I):
- wherein A and B each independently have Formula (a),
- wherein R1 and R2 each independently is —OH or —NH2;
- m, n, p and q each independently is 0, 1, 2, 3, or 4 and the sum of m+n is not zero.
3-5. (canceled)
A method for preparing a compound, the method comprising performing an azo coupling reaction between an aryl diazonium compound and a naphthalene derivative having a sulfonic acid group, thereby coupling the aryl diazonium compound to the naphthalene derivative to form the compound.
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11-15. (canceled)
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21-22. (canceled)
A kit comprising a first container comprising o-dianisidine bis(diazotized) zinc double salt, a second container comprising a naphthalene derivative having a sulfonic acid group, and an instruction.
26-30. (canceled)
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38-39. (canceled)
A peptide comprising the amino acid sequence set forth as:
- YRCMISYGGADYKRITV
- (SEQ ID NO:
- 1)
- or variant thereof;
- CYRAMISYGGADYKRITC
- (SEQ ID NO:
- 2)
- or variant thereof;
- LKYDAPAFTVT
- (SEQ ID NO:
- 3)
- or variant thereof;
- CLKYDAPAFTVTC
- (SEQ ID NO:
- 4)
- or variant thereof;
- LNWYRMSPSNQTDKLAA
- (SEQ ID NO:
- 5)
- or variant thereof;
- LNWYRMSPSNQTDKLAA
- (SEQ ID NO:
- 6)
- or variant thereof;
- CLNWYRMSPSNQTDKLAAC
- (SEQ ID NO:
- 7)
- or variant thereof;
- AISLAPKAQIK
- (SEQ ID NO:
- 8)
- or variant thereof;
- or
- CAISLAPKAQIKC
- (SEQ ID NO:
- 9)
- or variant thereof.
A peptide comprising the amino acid sequence set forth as:
- KVTLV
- (SEQ ID NO:
- 10)
- or variant thereof;
- RDDISEIQSLASDHSGR
- (SEQ ID NO:
- 11)
- or variant thereof;
- KEGLEEGDQILRV
- (SEQ ID NO:
- 12)
- or variant thereof;
- KFPAYER
- (SEQ ID NO:
- 13)
- or variant thereof;
- or
- KHALLDVTPNAVDR
- (SEQ ID NO:
- 14)
- or variant thereof
43-45. (canceled)
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49-52. (canceled)
54-55. (canceled)
Dependencies are inferred from the claim text.