TECHNIQUE FOR CREATING ANTIGEN-SPECIFIC REGULATORY T CELLS (Treg) IN WHICH EFFECTOR T CELL (Teff) ANTIGEN RECEPTORS ARE USED

A method of producing a regulatory T cell specific to an antigen, comprising:

• determining a TCR repertoire in an effector T cell population specific to the antigen in an effector T cell donor, comprising unbiasedly amplifying a TCR gene;
• identifying a pair of TCRα and TCRβ in the effector T cell population;
• checking whether the identified pair of TCRα and TCRβ has affinity to an antigen;
• cloning a full or partial nucleic acid sequence of TCRα and a full or partial nucleic acid sequence of TCRβ in the identified pair of TCRα and TCRβ;
• removing an endogenous TCR gene of a regulatory T cell;
• and
• introducing the cloned full or partial nucleic sequence of TCRα and full or partial nucleic acid sequence of TCRβ into the regulatory T cell so that the TCRα and the TCRβ are expressed as a pair.