Bioactive peptides derived from snakes
AssigneesUniversiteit Leiden, Academisch Ziekenhuis Leiden (H.O.D.N. Lumc)
InventorsHarald Martijn Ijsbrand Kerkkamp, Michael Keith Richardson, Gilles Philippus van Wezel, Jan Wouter Drijfhout, Robert Alexander Cordfunke, Michella Manon Voet, Petrus Hendricus Nibbering
Life of this patent
Estimating the term…
Abstract
The disclosure concerns snake-derived peptides and the useful application of such peptides to inhibit bacterial, fungal, viral and parasitic infections. The disclosed peptides are also useful for the treatment of an inflammatory condition or cancers.
Claims18
An isolated or recombinant peptide having between 20-200 amino acids, said peptide comprising:
- a) at least 20 consecutive amino acids of an amino acid sequence selected from the group consisting of:
- (SP27)
- (SEQ ID NO:
- 25)
- KRFKKFFRKVKKGVHRYFKKNKFYIAATIPYYG,
- (SP11)
- (SEQ ID NO:
- 10)
- KRFKKFFRKLKKSVKKRVKKFFKKPRVIGVTIPF,
- and
- (SP23)
- (SEQ ID NO:
- 21)
- KRFKKFFKKIKNSVKKRVKKFFKKPRVIGVSIPF;
- b) at least 20 consecutive amino acids of a retro-inverso sequence of a);
- or
- c) a functional variant of a) or b), wherein the variant:
- has one or more substitutions of an amino acid by a corresponding non-natural amino acid;
- has one or more substitutions of an amino acid by a corresponding D-amino acid;
- and/or
- has up to 5 amino acid substitutions.
An isolated or recombinant peptide having between 20-200 amino acids, a nucleic acid molecule encoding said peptide, or a composition comprising said peptide or said nucleic acid, wherein said peptide comprises:
- a) at least 20 consecutive amino acids of the amino acid sequence
- (SP4)
- (SEQ ID NO:
- 4)
- KRFKKFFKKLKNNVKKRVKKFFRKPRVIGVTIPF;
- b) at least 20 consecutive amino acids of a retro-inverso sequence of a);
- or
- c) a functional variant of a) or b), wherein the variant:
- has one or more substitutions of an amino acid by a corresponding non-natural amino acid;
- has one or more substitutions of an amino acid by a corresponding D-amino acid;
- and/or
- has up to 5 amino acid substitutions.
A method of using an isolated or recombinant peptide having between 20-200 amino acids to inhibit biofilm formation or to degrade biofilms as an anti-bacterial agent, anti-fungal agent, antiviral agent, anti-parasitic agent, as a diagnostic, crop protectant, disinfectant, preservative, anti-inflammatory agent, immune-modulating agent and/or anti-tumor agent, wherein said peptide comprises:
- a) at least 20 consecutive amino acids of the amino acid sequence
- (SP4)
- (SEQ ID NO:
- 4)
- KRFKKFFKKLKNNVKKRVKKFFRKPRVIGVTIPF;
- b) at least 20 consecutive amino acids of a retro-inverso sequence of a);
- or
- c) a functional variant of a) or b), wherein the variant:
- has one or more substitutions of an amino acid by a corresponding non-natural amino acid;
- has one or more substitutions of an amino acid by a corresponding D-amino acid;
- and/or
- has up to 5 amino acid substitutions.
An isolated or recombinant peptide having up to 200 amino acids, said peptide:
- a) comprising at least 60% of an amino acid sequence selected from the group consisting of:
- SP1
- (SEQ ID NO:
- 1)
- RKFKKSFKKIRKWVKKATKIAGEVAVEILITKVILGSVGL,
- SP2
- (SEQ ID NO:
- 2)
- KRVKRFKKFFKKVKKSVKKRLKKIFKKPMVIGVTIPF,
- SP3
- (SEQ ID NO:
- 3)
- KRFKKFFKKLKNSVKKRVKKFFRKPRVIGVTFPF,
- SP5
- (SEQ ID NO:
- 5)
- KRFKKFFRKLKKSVKKRAKEFFKKPRVIGVSIPF,
- SP6
- (SEQ ID NO:
- 6)
- KRFKKFFKKVKKSVKKRLKKIFKKPMVIGVTIPF,
- SP7
- (SEQ ID NO:
- 7)
- KRFKKFFKKVKKSVKKRLKKIFKKPMVIGVSIPF,
- SP8
- (SEQ ID NO:
- 8)
- KRFRKFFKRLLKSVRRAVKKFRKKPRLIGLSTLL,
- SP9
- (SEQ ID NO:
- 9)
- KRFKKFFKKVKKSVKKRLKKIFKKPMVIGVTFPF,
- SP12
- (SEQ ID NO:
- 11)
- KRFKKFFMKLKKSVKKRVMKFFKKPMVIGVTFPF,
- SP14
- (SEQ ID NO:
- 12)
- KRFKKFFKKAKKSVKKRLKKFFNKTRVIGVSIPF,
- SP15
- (SEQ ID NO:
- 13)
- KRFKKFFRKIKKGFRKIFKKTKIFIGGTIPI,
- SP16
- (SEQ ID NO:
- 14)
- KRFQNFFRELEKKFREFFRVYRITIGATIRF,
- SP17
- (SEQ ID NO:
- 15)
- KRFKDFFHRIRDGVRDFFRNNHFVCGVNFRF,
- SP18
- (SEQ ID NO:
- 16)
- KRFKKFFKKIKTGIKKVIKKTKQAIASHFRW,
- SP19
- (SEQ ID NO:
- 17)
- KRFLRVFKKLKRGIRKLFKKKKIVTGPLTS,
- SP20
- (SEQ ID NO:
- 18)
- AREKSFLQKMKVILQLSSQDQIQAF,
- SP21
- (SEQ ID NO:
- 19)
- KRFAGFFQFVIGVSFHF,
- SP22
- (SEQ ID NO:
- 20)
- DQVERGNFAKDGSKARVKKSLTKWVKFLKKIIAKLGRPLIGF,
- SP24
- (SEQ ID NO:
- 22)
- KRFKKFFKKLKNSVRKCAKKFFKKLRVIRVSIPF,
- SP25
- (SEQ ID NO:
- 23)
- KRFKKFFKKVKKSVKKRLKKIFKKPIVIGVSIPF,
- SP26
- (SEQ ID NO:
- 24)
- KRFTFYKKVKKNVENLLNKFFKKLRVIGDSIPF,
- SP28
- (SEQ ID NO:
- 26)
- KRFLRLVVFKKLKKGLRKLFKKKKIVTGHVTS,
- SP29
- (SEQ ID NO:
- 27)
- KRFKNFFKKIRDGIHEFIHNNRFVIGVNFRF,
- SP30
- (SEQ ID NO:
- 28)
- KRFKKFFKKIKNGIKTFIKKTQMAIGSHFRW,
- SP31
- (SEQ ID NO:
- 29)
- KRFKNFFKRVRDGIRDFFRKNHIVIGVNFRF,
- SP32
- (SEQ ID NO:
- 30)
- KRFLRVFRKLKKGLKKLFKRKKVVTGYVTA,
- SP33
- (SEQ ID NO:
- 31)
- KRFAGFFQFVVGVSFRF,
- SP34
- (SEQ ID NO:
- 32)
- KRFVGSFQLVVGVSFRF,
- and
- SP35
- (SEQ ID NO:
- 33)
- KRFKKFLKKLKKSVKKHVKEFFKKPRVIGV;
- b) comprising at least 60% of consecutive amino acids of a retro-inverso sequence of a);
- or
- c) a functional variant of a) or b), wherein the variant:
- has one or more substitutions of an amino acid by a corresponding non-natural amino acid;
- has one or more substitutions of an amino acid by a corresponding D-amino acid;
- and/or
- has up to 5 amino acid substitutions.
Dependencies are inferred from the claim text.