Methods For Increasing The Productivity Of A Filamentous Fungal Cell In The Production Of A Polypeptide

A method for constructing a mutant of a parent filamentous fungal cell, comprising modifying one or more genes each encoding a transcription factor in the parent filamentous fungal cell to produce the mutant, wherein the parent filamentous fungal cell or the mutant thereof comprises a coding sequence of a polypeptide of interest under the transcriptional control of a promoter regulated by one or more of the transcription factors, wherein the one or more transcription factors are selected from the group consisting of:

• (a) a transcription factor comprising an amino acid sequence having at least 70%, at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO:
• 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, or 124;
• (b) a transcription factor encoded by a polynucleotide comprising a nucleotide sequence having at least 70%, at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO:
• 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, or 123;
• and
• (c) a transcription factor encoded by a polynucleotide that hybridizes under high or very stringency conditions with the full-length complement of SEQ ID NO:
• 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, or 123;
• wherein the one or more transcription factor genes are modified in the parent filamentous fungal cell to produce the mutant rendering the mutant partially or completely deficient in the production of the one or more transcription factors, wherein (i) the modification of the one or more transcription factor genes increases the productivity of the mutant in the production of the polypeptide of interest when cultivated under the same conditions as the parent filamentous fungal cell without the modification of the one or more transcription factor genes, (ii) the modification of the one or more transcription factor genes reduces or eliminates the cellulase-negative phenotype in the resulting mutant compared to the parent filamentous fungal cell without the modification of the one or more transcription factor genes, or (iii) the modification of the one or more transcription factor genes results in a combination of (i) and (ii);
• and optionally recovering the mutant.

An isolated transcription factor, selected from the group consisting of:

• (a) a transcription factor comprising an amino acid sequence having at least at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO:
• 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, or 124;
• (b) a transcription factor encoded by a polynucleotide comprising a nucleotide sequence having at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to SEQ ID NO:
• 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, or 123;
• and
• (c) a transcription factor encoded by a polynucleotide that hybridizes under high or very high stringency conditions with the full-length complement of SEQ ID NO:
• 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, or 123.