Recombinant strains and medium formulation for enhancing secretion titer using a type iii secretion system
AssigneeNorthwestern University
InventorsDanielle Tullman Ercek, Lisa A. Burdette, Han Teng Wong
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Abstract
The present disclosure provides a recombinant Salmonella strain having a Type III secretion system (T3SS) comprising mutation which enhance protein expression and production. Additionally, methods and kits for using the recombinant Salmonella strain for producing a protein of interest are provided. Additionally, an optimized medium that increases protein expression in a Salmonella strain having a Type III secretion system (T3SS) is provided.
Claims28
A recombinant Salmonella strain having a Type III secretion system (T3 SS) comprising
- (a) a deletion or inactivation of hilE;
- or
- (b) an insertion of a protein coding sequence at the beginning of the hilD 3′UTR suitable to increase hilD expression in the strain;
- or
- (c) both (a) and (b).
(canceled)
(canceled)
A defined culture medium for increasing protein secretion comprising:
- about 10 g/L peptone,
- about 5 g/L yeast extract,
- glycerol or glucose in an amount effective to increase the osmolarity of the medium, and
- about 80 mM to about 120 mM potassium phosphate pH 7.4.
28-29. (canceled)
Dependencies are inferred from the claim text.