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US 2022/0064209 A1Published application
Methods of purifying polypeptides
AssigneeGenentech, Inc.
InventorsHui F. LIU, Brian David KELLEY, Deanna E. MYERS, Beth MCCOOEY, Krista Marie PETTY
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Abstract
The present invention provides methods for purifying a polypeptide from a composition comprising the polypeptide and at least one contaminant and formulations comprising the polypeptide purified by the methods. The methods for purifying include cation exchange material and/or mixed mode material.
Claims30
A method for purifying a antibody or immunoadhesin from a composition comprising the antibody or immunoadhesin and at least one contaminant, wherein the method comprises either (i) or (ii):
- (i) sequential steps of (a) loading the composition onto a cation exchange material at a loading density of greater than about 150 g/L of cation exchange material;
- and (b) loading a composition recovered from the cation exchange material onto a mixed mode material;
- or
- (ii) sequential steps of (a) loading the composition onto a mixed mode material;
- and (b) loading a composition recovered from mixed mode material onto a cation exchange material at a loading density of greater than about 150 g/L of cation exchange material;
- wherein the cation exchange material is resin particles.
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Dependencies are inferred from the claim text.