Genetically modified microorganism and method both for producing nicotinamide derivative, and vector for use in same
AssigneesTeijin Limited, Synart Co., Ltd.
InventorsShinichiro SHOJI, Jun ISHII, Akihiko KONDO, Hidekazu WATANABE, Masanobu KANOU, Ryota NAKAJIMA
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Abstract
Provided is a technique for synthesizing a nicotinamide derivative (NAm derivative) such as a nicotinamide mononucleotide (NMN) with high efficiency. A genetically modified microorganism is used, which can express, as nicotinamide phosphoribosylt ransferase (NAMPT), NAMPT having a conversion efficiency of 5-folds or more that of human NAMPT.
Claims16
A recombinant microorganism for producing a nicotinamide derivative, wherein the microorganism has been engineered to express a nicotinamide phosphoribosyl transferase (NAMPT), which converts nicotinamide and phosphoribosyl pyrophosphate into nicotinamide mononucleotide, and/or has been transformed with a vector carrying a nucleic acid encoding the amino acid sequence of the NAMPT, wherein the conversion efficiency of the NAMPT from nicotinamide to nicotinamide mononucleotide is five times or more of the conversion efficiency of a human NAMPT.
A vector carrying a nucleic acid encoding the amino acid sequence of nicotinamide phosphoribosyl transferase (NAMPT), which converts nicotinamide and phosphoribosyl pyrophosphate into nicotinamide mononucleotide, wherein the nucleic acid comprises a base sequence with a sequence identity of 80% or more with the base sequence represented by SEQ ID NO:2 or SEQ ID NO:5.
A vector carrying a nucleic acid encoding the amino acid sequence of a niacin transporter, which promotes cellular uptake of nicotinic acid and/or nicotinamide, wherein the nucleic acid comprises a base sequence with a sequence identity of 80% or more with the base sequence represented by SEQ ID NO:8 or SEQ ID NO:11.
A vector carrying a nucleic acid encoding the amino acid sequence of a nicotinamide derivative transporter, which promotes extracellular excretion of a nicotinamide derivative, wherein the nucleic acid comprises a base sequence with a sequence identity of 80% or more with the base sequence represented by SEQ ID NO:14.
Dependencies are inferred from the claim text.