Methods of forming bone interface scaffolds
AssigneeSparta Biopharma Inc.
InventorsIan N. ASKILL, Dushyanth R. SURAKANTI, Adam B. YANKE, Adam W. MARTINEZ, Kurt W.S. KLITZKE, Travis E. ENTROP, Dimitrios J. ANGELIS
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Abstract
Methods of forming a connective tissue-to-bone interface scaffolds (e.g., ligament-to-bone interface scaffolds, tendon-to-bone interface scaffolds, etc.). These scaffolds (grafts) may be formed from in such a way as to provide both a mineralized and demineralized layer in which the entire graft is flexible, compressible and compliant.
Claims30
A method of forming a compressible and compliant graft from a porous bone, the method comprising:
- cutting donor bone tissue into a thin layer having a thickness;
- applying a masking agent to a first side of a body of the donor bone tissue to a thickness of less than 1.5 mm, forming a masked portion and an unasked portion, wherein the masking agent comprises a cyanoacrylate having a polar side chain including an oxygen;
- demineralizing the unmasked portion of the donor bone tissue;
- and
- removing the masking agent to form a graft comprising a first layer of demineralized bone extending a length and a width of the body;
- and a second layer of mineralized bone that is continuously adjacent to the first layer, wherein the second layer has a thickness that is less than 1.5 mm.
A method of forming a compressible and compliant graft, the method comprising:
- cutting donor bone tissue into a thin layer having a thickness of less than 5.5 mm;
- removing organic material from the cut donor bone tissue;
- applying a masking agent to a first side of the donor bone tissue to a thickness of less than 1.5 mm, forming a masked portion and an unmasked portion, wherein the masking agent comprises an ethoxyethyl cyanoacrylate;
- demineralizing the unmasked portion of the donor bone tissue;
- and
- removing the masking agent to form a graft comprising a body having a length, a width and a thickness that is less than half the length and half the width, the body further comprising:
- a first layer of demineralized bone extending the length and the width of the body;
- and a second layer of mineralized bone that is continuously adjacent to the first layer, wherein the second layer has a thickness that is less than 1.5 mm.
A method of forming a compressible and compliant graft from a porous bone, the method comprising:
- applying a masking agent to a first side of a body comprising a donor bone tissue to a thickness of less than 1.5 mm, wherein the masking agent comprises a cyanoacrylate having a polar side chain including an oxygen;
- demineralizing an unmasked second side of the donor bone tissue, wherein the second side is opposite and adjacent the first side;
- and
- removing the masking agent to form a graft comprising a first layer of demineralized bone extending a length and a width of the body;
- and a second layer of mineralized bone that is continuously adjacent to the first layer, wherein the second layer has a thickness that is less than 1.5 mm.
Dependencies are inferred from the claim text.