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AssigneeGlaxosmithkline Biologicals SA
InventorsRainer FOLLADOR, Stefan Jochen KEMMLER, Michael Thomas KOWARIK, Gerald Johann POSCH
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Carrier proteins modified to incorporate one or more pilin glycotags and applications thereof for O-linked glycosylation are provided. In particular, a modified carrier protein comprising a carrier protein that comprises at least one GlycoTag, wherein the at least one GlycoTag is a Neisseria gonorrhoeae PglL GlycoTag (NgGlycoTag), Neisseria lactamica PglL GlycoTag (NlGlycoTag), or Neisseria shayeganii GlycoTag (NsGlycoTag), or combinations thereof is provided, together with nucleic acids and vectors encoding the modified carrier protein, host cells comprising these modofoed carrier proteins or nucleic acids encoding them, bioconjugates, methods of making bioconjugates and uses of the bioconjugates.
1-24. (canceled)
A modified carrier protein comprising a carrier protein that comprises at least one GlycoTag, wherein the at least one GlycoTag is a Neisseria gonorrhoeae PglL GlycoTag (NgGlycoTag), Neisseria lactamica PglL GlycoTag (NlGlycoTag), or Neisseria shayeganii GlycoTag (NsGlycoTag), or combinations thereof.
A modified carrier protein comprising a carrier protein that comprises at least one GlycoTag, wherein the at least one GlycoTag is a Neisseria meningitidis PglL GlycoTag (NmGlycoTag) consisting of a peptide sequence that is 12 to 19 amino acids long and comprises therein the sequence SEQ ID NO:
A modified carrier protein, characterized by a Pseudomonas aeruginosa exotoxin A (EPA) carrier protein comprising at least one Neisseria meningitidis PglL GlycoTag (NmGlycoTag), wherein the at least one NmGlycoTag is located at, with respect to SEQ ID NO:
Dependencies are inferred from the claim text.