Development of an edible vaccine
AssigneeKing Abdulaziz University
InventorsSayed Sartaj SOHRAB, Esam Ibraheem Ahmed ASHAR, Sherif Aly Abdelkhalek ELKAFRAWY, Ayman Talaat Abbas Abdelhadi, Edward P. RYBICKI, Emmanuel Aubrey MARGOLIN
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Abstract
Plant-based, edible vaccines are provided. The vaccines are or are made from plants that are genetically engineered to express antigens of disease-causing microbes, for example, antigens of the MERS-CoV virus, such as the S1 subunit of the spike protein.
Claims5
-7. (canceled)
A method of eliciting a mucosal immune response to Middle East Respiratory Syndrome coronavirus (MERS-CoV) in a subject in need thereof, said method comprising
- feeding the subject an edible wheat plant or a part of the edible wheat plant selected from the group consisting of a protoplast, a cell, a tissue, an organ, a cutting, an explant, a vegetative tissue, biomass, an inflorescence, a flower, a sepal, a petal, a pistil, a stigma, a style, an ovary, an ovule, an embryo, a receptacle, a seed, a stamen, an anther, a male or female gametophyte, a pollen grain, a meristem, a leaf, a stem and a root, wherein the edible wheat plant or the part of the edible wheat plant is genetically engineered to contain and express a nucleic acid sequence encoding sub-unit 1 of the MERS-CoV spike glycoprotein (S1 MERS-CoV),
- wherein the edible wheat plant or the part of the edible wheat plant is orally ingested and the S1 MERS-CoV is released from bioencapsulation in an intestine and taken up into M cells and induces the mucosal immune response;
- and
- wherein the subject is a camel.
9-10. (canceled)
A method of controlling spread of Middle East Respiratory Syndrome coronavirus (MERS-CoV) infection to humans by immunization of camels in an environment which includes humans and camels in proximity, comprising the steps of
- feeding an edible wheat plant or a part of the edible wheat plant selected from the group consisting of a protoplast, a cell, a tissue, an organ, a cutting, an explant, a vegetative tissue, biomass, an inflorescence, a flower, a sepal, a petal, a pistil, a stigma, a style, an ovary, an ovule, an embryo, a receptacle, a seed, a stamen, an anther, a male or female gametophyte, a pollen grain, a meristem, a leaf, a stem and a root expressing sub-unit 1 of the MERS-CoV spike glycoprotein (S1 MERS-CoV) to the camels;
- inducing a mucosal immunogenic response in the camels after release from bioencapsulation in the camels' intestines and absorbance in intestinal M cells in the absence of an adjuvant, and
- breaking a chain of transmission of MERS-CoV from the camels to humans.
A method of eliciting a mucosal immune response to Middle East Respiratory Syndrome coronavirus (MERS-CoV) in a camel in need thereof, said method comprising
- feeding the camel an edible vaccine consisting of a wheat plant or at least one part of the wheat plant selected from the group consisting of a protoplast, a cell, a tissue, an organ, a cutting, an explant, a vegetative tissue, biomass, an inflorescence, a flower, a sepal, a petal, a pistil, a stigma, a style, an ovary, an ovule, an embryo, a receptacle, a seed, a stamen, an anther, a male or female gametophyte, a pollen grain, a meristem, a leaf, a stem and a root, wherein the wheat plant or the part of the wheat plant is genetically engineered to contain and express a nucleic acid sequence encoding sub-unit 1 of the MERS-CoV spike glycoprotein (S1 MERS-CoV),
- wherein the vaccine is orally ingested and the S1 MERS-CoV is released from bioencapsulation in the camel's intestine and taken up into M cells and induces the mucosal immune response.
Dependencies are inferred from the claim text.